Toll-Like Receptors

Methods and Protocols

  • Claire E. McCoy
  • Luke A. J. O’Neill

Part of the Methods in Molecular Biology™ book series (MIMB, volume 517)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Methods to Detect and Analyze Toll-Like Receptors

  3. Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation

    1. Front Matter
      Pages 89-89
    2. Kiva Brennan, Caroline A. Jefferies
      Pages 91-104
    3. Nadia Ben Larbi, Caroline Jefferies
      Pages 105-132
    4. Peter Ulrichts, Irma Lemmens, Delphine Lavens, Rudi Beyaert, Jan Tavernier
      Pages 133-144
    5. Kelly Verhelst, Lynn Verstrepen, Beatrice Coornaert, Isabelle Carpentier, Rudi Beyaert
      Pages 205-215
  4. Genetic Techniques in Toll-Like Receptor Analysis

  5. Toll-Like Receptors and Disease

    1. Front Matter
      Pages 311-311
    2. Ruth Ferstl, Stephan Spiller, Sylvia Fichte, Stefan Dreher, Carsten J. Kirschning
      Pages 313-327
    3. Fabia Brentano, Diego Kyburz, Steffen Gay
      Pages 329-343
    4. Ryan Ungaro, Maria T. Abreu, Masayuki Fukata
      Pages 345-361
    5. Melissa B. Uccellini, Ana M. Avalos, Ann Marshak-Rothstein, Gregory A. Viglianti
      Pages 363-380
    6. Philip Smith, Niamh E. Mangan, Padraic G. Fallon
      Pages 401-413
  6. Back Matter
    Pages 441-447

About this book


In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories.  In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls.

Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.


Activation Antigen Immune System Immunity Microarray Proteomics genes molecular biology neutrophils protein complexes siRNA signal transduction

Editors and affiliations

  • Claire E. McCoy
    • 1
  • Luke A. J. O’Neill
    • 1
  1. 1.School of Biochemistry and ImmunologyTrinity College DublinDublin 2Ireland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-72-5
  • Online ISBN 978-1-59745-541-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site