DNA Microarrays for Biomedical Research

Methods and Protocols

  • Martin  Dufva
Part of the Methods in Molecular Biology book series (MIMB, volume 529)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Martin Dufva
    Pages 1-22
  3. Daniel A. Peiffer, Kevin L. Gunderson
    Pages 51-61
  4. Martin Dufva
    Pages 63-79
  5. Sascha Todt, Dietmar H. Blohm
    Pages 81-100
  6. Annelie Waldén, Peter Nilsson
    Pages 101-113
  7. Michael Stangegaard
    Pages 133-145
  8. Jesper Petersen, Lena Poulsen, Martin Dufva
    Pages 147-155
  9. Lena Poulsen, Jesper Petersen, Martin Dufva
    Pages 157-170
  10. Steve Blair, Layne Williams, Justin Bishop, Alexander Chagovetz
    Pages 171-196
  11. Kevin L. Gunderson
    Pages 197-213
  12. Baochuan Lin, Anthony P. Malanoski
    Pages 231-257
  13. Richard Redon, Diane Rigler, Nigel P. Carter
    Pages 259-266
  14. Richard Redon, Tomas Fitzgerald, Nigel P. Carter
    Pages 267-278
  15. Mickaël Durand-Dubief, Karl Ekwall
    Pages 279-295
  16. Back Matter
    Pages 297-304

About this book

Introduction

DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology.

Keywords

Allele DNA Gene expression analysis Genomic hybridization Micoarray fabrication Microarray Mutation analysis SNP Single Nucleotide Polymorphism gene expression genes hybridization mRNA expression studies molecular biology

Editors and affiliations

  • Martin  Dufva
  1. 1.Dept. Micro & NanotechnologyTechnical University of Denmark (DTU)Kongens LyngbyDenmark

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-538-1
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-69-5
  • Online ISBN 978-1-59745-538-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book