Patch-Clamp Methods and Protocols

  • Editors
  • Peter Molnar
  • James J. Hickman

Part of the Methods in Molecular Biology™ book series (MIMB, volume 403)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Pharmacology

    1. Front Matter
      Pages 1-1
    2. Edward R. Siuda, Jennifer C. Quirk, Eric S. Nisenbaum
      Pages 37-57
    3. Andrea Ghetti, António Guia, Jia Xu
      Pages 59-69
    4. Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter, Albrecht Lepple-Wienhues
      Pages 71-85
    5. Christine Leisgen, Mike Kuester, Christoph Methfessel
      Pages 87-109
  3. Physiology

    1. Front Matter
      Pages 111-111
    2. Gerhard Rammes, Matthias Eder, Walter Zieglgänsberger, Hans-Ulrich Dodt
      Pages 113-122
    3. Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, James J. Hickman
      Pages 165-173
    4. Peter Molnar, James J. Hickman
      Pages 175-184
    5. Sandrine Pouvreau, Claude Collet, Bruno Allard, Vincent Jacquemond
      Pages 185-194
    6. Alan D. Dorval II, Jonathan Bettencourt, Theoden I. Netoff, John A. White
      Pages 219-231
    7. Géza Berecki, Jan G. Zegers, Ronald Wilders, Antoni C. G. Van Ginneken
      Pages 233-250
  4. Biophysics

    1. Front Matter
      Pages 251-251

About this book

Introduction

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

Keywords

Aspartat Glutamat Peptide biophysics microscopy physiology skeletal muscle

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-529-9
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-698-6
  • Online ISBN 978-1-59745-529-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book