PCR Primer Design

  • Anton Yuryev

Part of the Methods in Molecular Biology™ book series (MIMB, volume 402)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Basic Principles and Software for PCR Primer Design

    1. Front Matter
      Pages 1-1
    2. Wojciech Rychlik
      Pages 35-59
    3. Thomas Kämpke
      Pages 75-91
    4. Jianping Huang, Anton Yuryev
      Pages 105-137
  3. Genome-Scale PCR Primer Design

    1. Front Matter
      Pages 139-139
    2. Dario Leister, Claudio Varotto
      Pages 141-157
    3. Gyan Prakash Srivastava, Dong Xu
      Pages 159-175
  4. Repeat Masking for PCR Primer Design

    1. Front Matter
      Pages 177-177
    2. Repeat Masking for PCR Primer Design

      1. Stefan Weckx, Peter De Rijk, Wim Glassee, Christine Van Broeckhoven, Jurgen Del-Favero
        Pages 178-199
      2. Reidar Andreson, Lauris Kaplinski, Maido Remm
        Pages 201-217
  5. Multiplex PCR Primer Design

    1. Front Matter
      Pages 219-219
    2. Multiplex PCR Primer Design

      1. Chaim Linhart, Ron Shamir
        Pages 220-244
      2. Richard Souvenir, Jeremy Buhler, Gary Stormo, Weixiong Zhang
        Pages 245-267
      3. Lars Kaderali
        Pages 269-285
      4. Lauris Kaplinski, Maido Remm
        Pages 287-303
      5. Rohan Fernandes, Steven Skiena
        Pages 305-314
  6. Allele-specific PCR

    1. Front Matter
      Pages 315-315
    2. Allele-specific PCR

      1. Michael Strerath, Ilka Detmer, Jens Gaster, Andreas Marx
        Pages 316-327
      2. Arun Apte, Siddharth Singh
        Pages 329-345
  7. Long PCR Primer Design

    1. Front Matter
      Pages 347-347
    2. Long PCR Primer Design

  8. DNA Methylation Mapping

    1. Front Matter
      Pages 369-369
    2. DNA Methylation Mapping

      1. Tamás Arányi, Gábor E. Tusnády
        Pages 385-402
      2. Owen Marshall
        Pages 403-414
  9. Back Matter
    Pages 415-431

About this book


In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.

In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists – geneticists, molecular biologists, molecular geneticists, and more – who routinely use PCR in their research.


Allele DNA Microarray PCR SNP molecular biology

Editors and affiliations

  • Anton Yuryev
    • 1
  1. 1.Application Science DepartmentAriadne Genomics Inc.RockvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-725-9
  • Online ISBN 978-1-59745-528-2
  • Series Print ISSN 1064-3745
  • Buy this book on publisher's site