Volume 1, Molecular and Genetic Methods

  • Scott Keeney

Part of the Methods in Molecular Biology book series (MIMB, volume 557)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Genetic Methods for Studying Meiotic Recombination and Chromosome Dynamics

  3. Molecular Analysis of Recombination and Protein-DNA Interactions During Meiosis

    1. Front Matter
      Pages 115-115
    2. Cyril Buhler, Robert Shroff, Michael Lichten
      Pages 143-164
    3. Jian Qin, Jaichandar Subramanian, Norman Arnheim
      Pages 165-181
    4. Steve D. Oh, Lea Jessop, Jessica P. Lao, Thorsten Allers, Michael Lichten, Neil Hunter
      Pages 209-234
    5. Kouji Hirota, Tomoyuki Fukuda, Takatomi Yamada, Kunihiro Ohta
      Pages 253-266
    6. Frédéric Baudat, Bernard de Massy
      Pages 305-322
    7. Liisa Kauppi, Celia A. May, Alec J. Jeffreys
      Pages 323-355
  4. Back Matter
    Pages 357-365

About this book


The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 1, Molecular and Genetic Methods details methods for culturing and manipulating commonly used model organisms and protocols for detecting and quantifying meiotic recombination events through genetic and physical analysis of DNA-DNA or Protein-DNA interactions. Methods are provided for studies in yeasts, plants, and animals, including mouse and human. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Meiosis: Volume 1, Molecular and Genetic Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.


Arabidopsis thaliana Budding and fission yeasts Chromosome dynamics DNA Meiotic recombination Protein-DNA interactions genes molecular biology mouse proteins saccharomyces cerevisiae

Editors and affiliations

  • Scott Keeney
    • 1
  1. 1.Molecular Biology ProgramMemorial Sloan-Kettering Cancer CenterNew YorkU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-934115-66-4
  • Online ISBN 978-1-59745-527-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site