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© 2007

Immunological Tolerance

Methods and Protocols

  • Paul J. Fairchild
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 380)

Table of contents

  1. Front Matter
    Pages i-xiv
    PDF

  2. Cell Types Contributing to Immunological Tolerance

    1. Front Matter
      Pages N1-N1
      PDF
    2. Frontiers of Immunological Tolerance
      Giorgio Raimondi, Hēeth R. Turnquist, Angus W. Thomson
      Pages 1-24
    3. Balancing Tolerance and Immunity
      Elena Shklovskaya, Barbara Fazekas de St. Groth
      Pages 25-46
    4. Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow
      Ludovica Bruno
      Pages 47-57
    5. Genetic Modification of Dendritic Cells Through the Directed Differentiation of Embryonic Stem Cells
      Paul J. Fairchild, Kathleen F. Nolan, Herman Waldmann
      Pages 59-72
    6. Generation of Immunocompetent T Cells from Embryonic Stem Cells
      Renée F. de Pooter, Juan Carlos Zúñiga-Pflücker
      Pages 73-81
    7. Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells
      Silvia Gregori, Rosa Bacchetta, Laura Passerini, Megan K. Levings, Maria Grazia Roncarolo
      Pages 83-105
    8. Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines
      Michiyuki Kasai, Toshiaki Mizuochi
      Pages 107-123

  3. The Study of Immunological Tolerance In Vitro

    1. Front Matter
      Pages N2-N2
      PDF
    2. Thymus Organogenesis and Development of the Thymic Stroma
      Craig S. Nowell, Alison M. Farley, C. Clare Blackburn
      Pages 125-162
    3. Generation of a Tissue-Engineered Thymic Organoid
      Fabrizio Vianello, Mark C. Poznansky
      Pages 163-170
    4. Studying T-Cell Repertoire Selection Using Fetal Thymus Organ Culture
      Philip G. Ashton-Rickardt
      Pages 171-184
    5. Investigating Central Tolerance With Reaggregate Thymus Organ Cultures
      Graham Anderson, Eric J. Jenkinson
      Pages 185-196
    6. Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles
      Marie-Lise Dion, Rafick-Pierre Sékaly, Rémi Cheynier
      Pages 197-213
    7. Gene Expression Profiling of Dendritic Cells by Microarray
      Maria Foti, Paola Ricciardi-Castagnoli, Francesca Granucci
      Pages 215-224
    8. SAGE Analysis of Cell Types Involved in Tolerance Induction
      Kathleen F. Nolan, Stephen P. Cobbold, Herman Waldmann
      Pages 225-251
    9. Analyzing the Physicodynamics of Immune Cells in a Three-Dimensional Collagen Matrix
      Peter Reichardt, Frank Gunzer, Matthias Gunzer
      Pages 253-269

  4. The Study of Immunological Tolerance In Vivo

    1. Front Matter
      Pages N3-N3
      PDF
    2. Etiology of Autoimmmune Disease
      Eli Sercarz, Claudia Raja-Gabaglia
      Pages 271-283
    3. Animal Models of Spontaneous Autoimmune Disease
      Nadia Giarratana, Giuseppe Penna, Luciano Adorini
      Pages 285-311
    4. Antigen-Based Therapy and Immune-Regulation in Experimental Autoimmune Encephalomyelitis
      Mandy J. McGeachy, Richard O’Connor, Leigh A. Stephens, Stephen M. Anderton
      Pages 313-326
    5. Induction and Regulation of Inflammatory Bowel Disease in Immunodeficient Mice by Distinct CD4+ T-Cell Subsets
      Kevin J. Maloy
      Pages 327-335
    6. In Vivo Models for the Study of Transplantation Tolerance
      Hannah Stewart, Rommel Ravanan, Richard Smith
      Pages 337-346
    7. Ectopic Transplantation of Tissues Under the Kidney Capsule
      Nathan J. Robertson, Paul J. Fairchild, Herman Waldmann
      Pages 347-353
    8. Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node
      Susanna Celli, Philippe Bousso
      Pages 355-363
    9. Tracing Tolerance and Immunity In Vivo by CFSE-Labeling of Administered Cells
      Elizabeth Ingulli
      Pages 365-376

  5. Methods for Inducing and Breaking Immunological Tolerance

    1. Front Matter
      Pages N4-N4
      PDF
    2. Thymic Involution
      Frances T. Hakim, Ronald E. Gress
      Pages 377-390
    3. Inducing Mixed Chimerism and Transplantation Tolerance Through Allogeneic Bone Marrow Transplantation With Costimulation Blockade
      Ines Pree, Thomas Wekerle
      Pages 391-403
    4. Induction of Dominant Tolerance Using Monoclonal Antibodies
      Ana Água-Doce, Luis Graça
      Pages 405-429
    5. Induction of Tolerance by Adoptive Transfer of Treg Cells
      Kanji Nagahama, Eiji Nishimura, Shimon Sakaguchi
      Pages 431-442
    6. Modulation of the Immune Response Using Dendritic Cell-Derived Exosomes
      Nicole R. Bianco, Seon-Hee Kim, Adrian E. Morelli, Paul D. Robbins
      Pages 443-455
    7. Breaking Self-Tolerance to Tumor-Associated Antigens by In Vivo Manipulation of Dendritic Cells
      Ines Mende, Edgar G. Engleman
      Pages 457-468
  6. Back Matter
    Pages 469-477
    PDF

About this book

Introduction

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.

Keywords

AIDS Antigen Immunologie Thymus Transplantation autoimmune disease gene therapy infectious infectious disease infectious diseases

Editors and affiliations

  • Paul J. Fairchild
    • 1
  1. 1.Sir William Dunn School of PathologyUniversity of OxfordOxfordUK

Bibliographic information

  • Book Title Immunological Tolerance
  • Book Subtitle Methods and Protocols
  • Editors Paul J. Fairchild
  • Series Title Methods in Molecular Biology™
  • DOI https://doi.org/10.1007/978-1-59745-395-0
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-652-8
  • Softcover ISBN 978-1-61737-696-2
  • eBook ISBN 978-1-59745-395-0
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 478
  • Number of Illustrations 61 b/w illustrations, 5 illustrations in colour
  • Topics Immunology
    Infectious Diseases
  • Buy this book on publisher's site

Reviews

From the reviews:

"Immunological Tolerance: Methods and Protocols unites the most recent ideas and techniques of outstanding scientists in this area. … I would recommended this book, not only as a way of getting into the field of immunological tolerance, but also as a laboratory resource for the techniques required. It provides a thorough guide to the field of immunological tolerance that will be helpful to both new and established scientists wishing to investigate this exciting field." (Carl Goodyear, Immunology News, February, 2008)

"This guide provides clear information on modern laboratory techniques that are appropriate for the subject at hand -- immunological tolerance. In addition there are reviews of the literature on tolerance. … The audience for this book includes both basic science and clinical researchers who are studying problems relating to immunological tolerance. Experts in the field write the various chapters. … Thus, for anyone interested in studying tolerance, this is a potentially useful source of information." (Marion C. Cohen, Doody’s Review Service, September, 2007)