Transgenic Wheat, Barley and Oats

Production and Characterization Protocols

  • Huw D. Jones
  • Peter R. Shewry

Part of the Methods in Molecular Biology™ book series (MIMB, volume 478)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Paul A. Lazzeri*, Huw D. Jones
      Pages 3-20
  3. Transformation and Regeneration

    1. Front Matter
      Pages 22-22
    2. Huw D. Jones*, Caroline A. Sparks
      Pages 23-37
    3. Alison Huttly
      Pages 39-69
    4. Caroline A. Sparks, Huw D. Jones*
      Pages 71-92
    5. Sujata Agarwal, Star Loar, Camille Steber, Janice Zale*
      Pages 105-113
    6. Thierry Risacher, Melanie Craze, Sarah Bowden, Wyatt Paul*, Tina Barsby
      Pages 115-124
    7. Wendy A. Harwood*, Mark A. Smedley
      Pages 125-136
    8. Wendy A. Harwood*, Joanne G. Bartlett, Silvia C. Alves, Matthew Perry, Mark A. Smedley, Nicola Leyl et al.
      Pages 137-147
    9. Shahina B. Maqbool*, Heng Zhong, Hesham F. Oraby, Mariam B. Sticklen
      Pages 149-168
  4. Gene and Protein Expression

    1. Front Matter
      Pages 170-170
    2. Huw D. Jones*, Caroline A. Sparks
      Pages 171-184
    3. Silvia Travella*, Beat Keller
      Pages 185-199
  5. Characterisation of Transgenic Plants

    1. Front Matter
      Pages 202-202
    2. Philippe Vain, Vera Thole
      Pages 203-226
    3. María Marcela Baudo*, Stephen J. Powers, Rowan A. C. Mitchell, Peter R. Shewry
      Pages 247-272

About this book

Introduction

The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture.  In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research.  Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants.  The in-depth chapters adhere to the highly successful Methods in Molecular Biology™ series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.

Keywords

Agrobacterium Biolistics Cereal research Embryo Flora Gene and protein expression Proteom Transformation and regeneration Transgenic plants genome wheat

Editors and affiliations

  • Huw D. Jones
  • Peter R. Shewry

There are no affiliations available

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-379-0
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-961-1
  • Online ISBN 978-1-59745-379-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book