Book Volume 371 2007

Biological Aging

Methods and Protocols

Editors:

ISBN: 978-1-58829-658-0 (Print) 978-1-59745-361-5 (Online)

Table of contents (25 protocols)

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  1. Front Matter

    Pages i-xv

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    Protocol

    Pages 1-7

    Techniques for Analysis of Biological Aging

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    Protocol

    Pages 9-19

    Aging Cell Culture

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    Protocol

    Pages 21-31

    Methods to Detect Biomarkers of Cellular Senescence

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    Protocol

    Pages 33-44

    Methods for Cell Sorting of Young and Senescent Cells

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    Protocol

    Pages 45-72

    Telomere Length Analysis

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    Protocol

    Pages 73-80

    A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems

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    Protocol

    Pages 81-87

    A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro

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    Protocol

    Pages 89-95

    The Chronological Life Span of Saccharomyces cerevisiae

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    Protocol

    Pages 97-109

    Caloric Restriction and Life Span Determination of Yeast Cells

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    Protocol

    Pages 111-141

    Methods for Nutrigenomics and Longevity Studies in Drosophila

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    Protocol

    Pages 143-149

    Caloric Restriction Mimetics

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    Protocol

    Pages 151-165

    Extension of Cell Life Span Using Exogenous Telomerase

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    Protocol

    Pages 167-178

    Inducing Cellular Senescence Using Defined Genetic Elements

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    Protocol

    Pages 179-189

    Methods of Cellular Senescence Induction Using Oxidative Stress

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    Protocol

    Pages 191-207

    Nuclear Transfer Methods to Study Aging

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    Protocol

    Pages 209-226

    Use of Ribozymes in Cellular Aging Research

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    Protocol

    Pages 227-236

    Methods of Evaluating the Effect of Pharmacological Drugs On Aging and Life Span in Mice

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    Protocol

    Pages 237-248

    Identifying Genes that Extend Life Span Using a High-Throughput Screening System

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    Protocol

    Pages 249-265

    Application of DNA Microarray Technology to Gerontological Studies

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    Protocol

    Pages 267-287

    Detection and Analysis of Somatic Mutations at a lacZ Reporter Locus in Higher Organisms

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