Kidney Research

Experimental Protocols

  • Gavin J. Becker
  • Tim D. Hewitson

Part of the Methods in Molecular Biology book series (MIMB, volume 466)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Isolation and Propagation of Cell Populations

    1. Front Matter
      Pages 1-1
    2. Paolo Menè, Antonella Stoppacciaro
      Pages 1-15
    3. David A. Vesey, Weier Qi, Xinming Chen, Carol A. Pollock, David W. Johnson
      Pages 19-24
    4. Lauren Grimwood, Rosemary Masterson
      Pages 25-37
  3. Animal Models

    1. Front Matter
      Pages 1-1
    2. Tim D. Hewitson, Takahiko Ono, Gavin J. Becker
      Pages 41-57
  4. Techniques and Applications

    1. Front Matter
      Pages 1-1
    2. Zhonghua Qi, Matthew D. Breyer
      Pages 61-72
    3. Robert P. Woroniecki, Erwin P. Bottinger
      Pages 73-82
    4. Chris Tikellis, Philip Koh, Wendy Burns, Phillip Kantharidis
      Pages 83-107
    5. A. Richard Kitching, Michael P. Kuligowski, Michael J. Hickey
      Pages 109-117
    6. Ian A. Darby, Alexis Desmoulière, Tim D. Hewitson
      Pages 119-132
    7. Tim D. Hewitson, Lauren Grimwood
      Pages 133-147
    8. Christian Rosenberger, Seymour Rosen, Alexander Paliege, Samuel N. Heyman
      Pages 161-174
    9. Eleni Giannakis, Chrishan S. Samuel, Wee-Ming Boon, Mary Macris, Tim D. Hewitson, John D. Wade
      Pages 237-249
    10. Yoshitsugu Takabatake, Yoshitaka Isaka, Enyu Imai
      Pages 251-263
  5. Back Matter
    Pages 265-268

About this book


As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment.  The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney.  In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.


Cell isolation Cell propagation In vivo and in vitro Laboratory Nephrology Renal cells Renal disease Termination electron microscopy gene expression genes molecular biology signal transduction tissue

Editors and affiliations

  • Gavin J. Becker
  • Tim D. Hewitson

There are no affiliations available

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-945-1
  • Online ISBN 978-1-59745-352-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site