Overview
- Editors:
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Raymond Tyther
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David Sheehan
- Covers a wide biological range of proteome sources including microbes, plants and animals
- Addresses key aspects of experimental design
- Includes some of the latest approaches such as DIGE and detection of post-translational modification
- Includes supplementary material: sn.pub/extras
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Table of contents (36 protocols)
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- Claus Zabel, Joachim Klose
Pages 311-338
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- Cécile Lelong, Mireille Chevallet, Sylvie Luche, Thierry Rabilloud
Pages 339-350
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- Rukhsana Sultana, Tanea Reed, D. Allan Butterfield
Pages 351-361
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- Sarah L. Cuddihy, James W. Baty, Kristin K. Brown, Christine C. Winterbourn, Mark B. Hampton
Pages 363-375
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- Sarka Beranova-Giorgianni, Dominic M. Desiderio, Francesco Giorgianni
Pages 383-396
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- Elisabetta Gianazza, Ivano Eberini, Pietro Ghezzi
Pages 397-415
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- Chad Walls, Bo Zhou, Zhong-Yin Zhang
Pages 417-429
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- Zhenjun Zhao, Pamela J. Russell
Pages 431-438
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- Brian P. Bradley, Bose Kalampanayil, Michael C. O’Neill
Pages 455-468
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- Bart Samyn, Kjell Sergeant, Jozef Van Beeumen
Pages 469-482
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- Kazuishi Kubota, Toshiyuki Kosaka, Kimihisa Ichikawa
Pages 483-494
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- Kjell Sergeant, Jozef Van Beeumen, Bart Samyn
Pages 495-506
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- Mai-Loan Huynh, Pamela Russell, Bradley Walsh
Pages 507-513
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- Patricia M. Palagi, Frédérique Lisacek, Ron D. Appel
Pages 515-531
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- Christine Hoogl, Khaled Mostaguir, Ron D. Appel
Pages 533-539
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Back Matter
Pages 541-545
About this book
The human genome and other large-scale genome sequencing projects have inevitably led to a focus on the proteins encoded by genes. The field of proteomics has grown enormously as a result and a number of high-throughput technologies have now been developed allowing discovery-led investigations of protein populations rather than more traditional hypothesis-led studies on single proteins. These high-throughput techno- gies include gene and protein microarrays, the yeast two-hybrid system, and various mass spectrometry methodologies. However, despite developments and improvements in these technologies, two-dimensional electrophoresis (2DE) remains one of the most widely used approaches. This technique was revolutionised by the development of immobilised pH gradient strips which are now commercially available. This has made possible highly reproducible separations of matched samples. Developments in staining, mass spectr- etry, and bioinformatics supported these developments and have led to a measure of standardisation in design, execution, and analysis of proteomics experiments. This book began life as a proposed update of the excellent volume 2DE Protocols edited by Andrew Link of the University of Washington at Seattle. However, we re- ised that 2DE has undergone major development in aspects of its technology in recent years and we were anxious to reflect these in the present volume. We are also conscious that many researchers have now begun to apply proteomics methodologies to a growing range of biological material and we were anxious to include contributions to reflect the challenges posed in sample preparation in less widely used organisms.