Two-Dimensional Electrophoresis Protocols

  • Raymond Tyther
  • David Sheehan

Part of the Methods in Molecular Biology book series (MIMB, volume 519)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Thierry Rabilloud
    Pages 19-30
  3. Mireille Starita-Geribaldi
    Pages 31-45
  4. Ben Herbert, Elizabeth Harry
    Pages 47-63
  5. Matthias Plöscher, Bernhard Granvogl, Veronika Reisinger, Axel Masanek, Lutz Andreas Eichacker
    Pages 65-82
  6. Brian McDonagh
    Pages 103-109
  7. Bettina Levänen, Åsa M. Wheelock
    Pages 112-129
  8. Philip Cash, Evelyn Argo
    Pages 131-144
  9. Pan-Young Jeong, Keun Na, Mi-Jeong Jeong, David Chitwood, Yhong-Hee Shim, Young-Ki Paik
    Pages 145-169
  10. Claus Zabel, Joachim Klose
    Pages 171-196
  11. Suze Chora, Maria Bebianno, Michèle Roméo
    Pages 197-204
  12. Anne von Zychlinski, Wilhelm Gruissem
    Pages 205-220
  13. Katrin Marcus, Cornelia Joppich, Caroline May, Kathy Pfeiffer, Barbara Sitek, Helmut Meyer et al.
    Pages 221-240
  14. Kelly Andringa, Adrienne King, Shannon Bailey
    Pages 241-258
  15. Kiichi Fukui, Susumu Uchiyama
    Pages 259-271
  16. Diana M. Wong, Khosrow Adeli
    Pages 273-289
  17. Won-A Joo, David Speicher
    Pages 291-304

About this book

Introduction

Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.

Keywords

Chromosome Polyacrylamid-Gelelektrophorese Proteomics Translation algorithms bioinformatics biological materials databases evolution molecular biology post-translational modification sequence analysis

Editors and affiliations

  • Raymond Tyther
  • David Sheehan

There are no affiliations available

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-281-6
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-937-6
  • Online ISBN 978-1-59745-281-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book