© 2009

Cancer Stem Cells

Methods and Protocols

  • John S. Yu

Part of the Methods in Molecular Biology book series (MIMB, volume 568)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Michael C. Dovey, Leonard I. Zon
    Pages 1-5
  3. Aniruddha J. Deshpande, Farid Ahmed, Christian Buske
    Pages 21-35
  4. Hugo Guerrero-Cázares, Kaisorn L. Chaichana, Alfredo Quiñones-Hinojosa
    Pages 73-83
  5. Hangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis et al.
    Pages 85-138
  6. Chenwei Li, C. J. Lee, Diane M. Simeone
    Pages 161-173
  7. Laurie Ailles, Mark Prince
    Pages 175-193
  8. Patrizia Tunici, John S. Yu
    Pages 195-201
  9. Yin Shen, Shaun D. Fouse, Guoping Fan
    Pages 203-216
  10. Serena Pellegatta, Gaetano Finocchiaro
    Pages 233-247
  11. Jennifer Katz, Joseph Krueger, Brunhilde Felding-Habermann, Evan Y. Snyder
    Pages 249-259
  12. Vera S. Donnenberg, E. Michael Meyer, Albert D. Donnenberg
    Pages 261-279
  13. Back Matter
    Pages 281-283

About this book


Through the revolutionary concept of cancer stem cells, cancer research has been reinvigorated to study the role of these unique cells in cancer propagation and as targets of innovative therapies. In Cancer Stem Cells: Methods and Protocols, preeminent researchers have compiled cancer stem cell research techniques and protocols to promote healthy competition, discourse, and collaboration in this vital field.  The volume covers extensive topics such as identification and isolation of cancer stem cells, animal models of cancer stem cells, methylation profiling, the contribution of the niche in the regulation of cancer stem cells, immunologic targeting, and the use of normal stem cells as a treatment, among other subjects.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Cancer Stem Cells: Methods and Protocols will aid scientists around the world in the furthering of our understanding of cancer initiation and propagation and, most importantly, in the development of novel targets for cancer therapy.


Brain cancer Carcinom DNA Head and neck cancer Leukemia Marker expression Neurospheres Pancreatic cancer Pituitary adenoma Prostate cancer Tumors molecular biology

Editors and affiliations

  • John S. Yu

There are no affiliations available

Bibliographic information

  • Book Title Cancer Stem Cells
  • Book Subtitle Methods and Protocols
  • Editors John S. Yu
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-938-3
  • Softcover ISBN 978-1-61779-753-8
  • eBook ISBN 978-1-59745-280-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XVI, 284
  • Number of Illustrations 42 b/w illustrations, 16 illustrations in colour
  • Topics Oncology
    Cancer Research
    Stem Cells
    Cell Culture
  • Buy this book on publisher's site


From the reviews:

“This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. … The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. … The topic is fascinating and clinically relevant.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)