Metabolomics

1. Front Matter

   Pages i-xii

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2. Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry

   1. Front Matter

      Pages 1-1

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   2. Metabolite Profiling in Blood Plasma

      • Oliver Fiehn, Tobias Kind

      Pages 3-17

   3. Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles

      • Alexander Erban, Nicolas Schauer, Alisdair R. Fernie, Joachim Kopka

      Pages 19-38

   4. Metabolomic Profiling of Natural Volatiles

      • Yury M. Tikunov, Francel W. A. Verstappen, Robert D. Hall

      Pages 39-53

3. Metabolomics: Data Integration and Data Mining

   1. Front Matter

      Pages 17-17

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4. Metabolomics: Data Integration and Data Mining

   1. Integrative Profiling of Metabolites and Proteins

      • Katja Morgenthal, Stefanie Wienkoop, Florian Wolschin, Wolfram Weckwerth

      Pages 57-75

   2. A Gentle Guide to the Analysis of Metabolomic Data

      • Ralf Steuer, Katja Morgenthal, Wolfram Weckwerth, Joachim Selbig

      Pages 105-126

5. Metabolomics: Data Integration and Data Mining

   1. Integrating Profiling Data

      • Ewa Urbanczyk-Wochniak, Lothar Willmitzer, Alisdair R. Fernie

      Pages 77-85

   2. Visualization and Analysis of Molecular Data

      • Matthias Scholz, Joachim Selbig

      Pages 87-104

6. Capillary Electrophoresis Coupled to Mass Spectrometry

   1. Front Matter

      Pages 37-37

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   2. Capillary Electrophoresis-Mass Spectrometry for Metabolomics

      • Tomoyoshi Soga

      Pages 129-137

7. Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation

   1. Front Matter

      Pages 55-55

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   2. Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics

      • Vladimir V. Tolstikov, Oliver Fiehn, Nobuo Tanaka

      Pages 141-155

8. Electrochemical Detection of Metabolites

   1. Front Matter

      Pages 75-75

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   2. High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors

      • Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah-Daouk, Wayne R. Matson

      Pages 159-174

9. Metabolic Fluxes

   1. Front Matter

      Pages 175-175

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   2. Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data

      • Annik Nanchen, Tobias Fuhrer, Uwe Sauer

      Pages 177-197

   3. Understanding the Roadmap of Metabolism by Pathway Analysis

      • Stefan Schuster, Axel von Kamp, Mikhail Pachkov

      Pages 199-226

10. NMR Metabolome Analysis

    1. Front Matter

       Pages 103-103

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Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

• Chromatography
• Mass Spectrometry
• Termination
• data mining
• metabolism

From the reviews:

"This book forms part of an extensive and successful series from Methods in Molecular Biology … . this book provides a good introduction to metabolomics, with useful protocols and relevant data analysis techniques that can be adapted easily to suit any researcher’s needs." (Elizabeth Want, ChemMedChem, Issue 2, 2007)

• Department of Metabolic Networks, Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

  Wolfram Weckwerth

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