© 2008

Prion Protein Protocols

  • Andrew F. Hill

Part of the Methods in Molecular Biology™ book series (MIMB, volume 459)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Cathryn L. Haigh, David R. Brown
    Pages 21-34
  3. Hanna Gyllberg, Kajsa Löfgren
    Pages 35-48
  4. Sukhvir P. Mahal, Cheryl A. Demczyk, Emery W. Smith Jr., Peter-Christian Klohn, Charles Weissmann
    Pages 49-68
  5. Giuseppe D. Ciccotosto, Roberto Cappai, Anthony R. White
    Pages 83-96
  6. Leonid Breydo, Natallia Makarava, Ilia V. Baskakov
    Pages 105-115
  7. Surachai Supattapone, Nathan R. Deleault, Judy R. Rees
    Pages 117-130
  8. Natallia Makarava, Ilia V. Baskakov
    Pages 131-143
  9. Sen Han, Andrew F. Hill
    Pages 145-159
  10. Jonathan D. F. Wadsworth, Caroline Powell, Jonathan A. Beck, Susan Joiner, Jacqueline M. Linehan, Sebastian Brandner et al.
    Pages 197-227
  11. Victoria Lewis, Genevieve M. Klug, Andrew F. Hill, Steven J. Collins
    Pages 241-247
  12. Glenn C. Telling
    Pages 249-263
  13. Back Matter
    Pages 275-280

About this book


While much information has been gained over the last two decades regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form. In Prion Protein Protocols, an international team of experts provide an up-to-date collection of current methods in this unique area of neuroscience.  The chapters contained in this volume, both protocols and useful review chapters, feature topics such as the cell biology of prions, techniques and approaches to studying prion infection in cultured cells, how these systems can be used as a rapid bioassay, and prion protein misfolding. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include readily reproducible, step-by-step laboratory protocols, lists of the necessary materials and reagents, and the Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Prion Protein Protocols is an ideal collection for researchers investigating the growing field of mammalian prion disease.


Bovine Spongiform Encepalopathy Creutzfeldt-Jakob Disease Infectious prions Mammalian prion disease Neuroscience Protein misfolding Rapid bioassay cell lines infection infectious molecular biology neurons

Editors and affiliations

  • Andrew F. Hill
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute; Department of Pathology and Mental Health Institute of VictoriaUniversity of MelbourneMelbourneAustralia

Bibliographic information


From the reviews:

"Interspersed are concise reviews focusing on current growth areas in the prion field. … The protocols are clear and authoritative. For those entering the field it would be an excellent guide." (Hugh Pennington, Microbiology Today, February, 2009)