Hepatocyte Transplantation

Methods and Protocols

  • Anil Dhawan
  • Robin D. Hughes

Part of the Methods in Molecular Biology book series (MIMB, volume 481)

Table of contents

  1. Front Matter
    Pages 1-17
  2. Juliana Puppi, Anil Dhawan
    Pages 1-16
  3. Ragai R. Mitry
    Pages 17-23
  4. Claire Terry, Robin D. Hughes
    Pages 25-34
  5. José V. Castell, María José Gómez-Lechón
    Pages 35-46
  6. Brian G. Lake, Roger J. Price, Amanda M. Giddings, David G. Walters
    Pages 47-58
  7. Mustapha Najimi, Françoise Smets, Etienne Sokal
    Pages 59-74
  8. Jurgen Seppen, Ebtisam El Filali, Ronald Oude Elferink
    Pages 75-82
  9. Anne Weber, Marie-Thérèse Groyer-Picard, Ibrahim Dagher
    Pages 83-96
  10. Robert A. Fisher, Valeria R. Mas
    Pages 97-105
  11. Xia Wang, Prashant Mani, Debi P. Sarkar, Namita Roy-Chowdhury, Jayanta Roy-Chowdhury
    Pages 117-139
  12. Rosemary Jeffery, Richard Poulsom, Malcolm R. Alison
    Pages 141-154
  13. Toshio Miki, Fabio Marongiu, Ewa C.S. Ellis, Ken Dorko, Keitaro Mitamura, Aarati Ranade et al.
    Pages 155-168
  14. Niloufar Safinia, Stephen L Minger
    Pages 169-180
  15. Yue Wu, Chetan C. Shatapathy, Stephen L. Minger
    Pages 181-191
  16. Ruth Joplin, Stivelia Kachilele
    Pages 193-206
  17. Michel Modo, Thomas J. Meade, Ragai R. Mitry
    Pages 207-219
  18. Back Matter
    Pages 229-231

About this book


Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.


Acute liver failure Animal models Human hepatocytes Liver-based metabolic disease Stem cells cryopreservation gene transfer genes molecular biology

Editors and affiliations

  • Anil Dhawan
    • 1
  • Robin D. Hughes
    • 2
  1. 1.King’s College HospitalLondonUK
  2. 2.School of MedicineKing’s College LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-172-1
  • Online ISBN 978-1-59745-201-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site