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  • © 2009

The Protein Protocols Handbook

Editors:

  • Offers over 200 key, readily reproducible protocols that ensure robust, successful results
  • Includes many new techniques for the study of post-translational modifications
  • Demonstrates the range of techniques available and their strengths and limitations
  • Contains numerous time-saving techniques for even the most highly skilled researchers

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (208 protocols)

  1. Electrophoresis of Proteins and Peptides and Dectection in Gels

    1. Separation of Proteins by Blue Native Electrophoresis (BN-PAGE)

      • Elke A. Dian, Joachim Rassow, Christian Motz
      Pages 201-210
    2. Separation of Proteins by Gel Electrophoresis in the Tris-Taurine-HCl System

      • Sylvie Luche, Mireille Chevallet, Cécile Lelong, Thierry Rabilloud
      Pages 211-219
    3. Serial Immobilized pH Gradient Isoelectric Focusing over pH 4–9

      • Slobodan Poznanović, Wojciech Wozny, Helmut Zengerling, Gerhard P. Schwall, Michael A. Cahill
      Pages 269-277
    4. Two-Dimensional PAGE of High-Molecular-Mass Proteins

      • Masamichi Oh-Ishi, Tadakazu Maeda
      Pages 299-304
    5. Casting Immobilized pH Gradients

      • Elisabetta Gianazza
      Pages 305-322
    6. Microchip Capillary Electrophoresis

      • Barbara A. Fogarty, Nathan A. Lacher, Susan M. Lunte
      Pages 329-359
    7. Protein Separations in Microfluidic Chips

      • Andrea W. Chow, Bahram Fathollahi
      Pages 361-378
    8. Difference Gel Electrophoresis (DIGE)

      • David B. Friedman, Kathryn S. Lilley
      Pages 379-408

About this book

Since the publication of the bestselling second edition of John Walker’s widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.

Each readily reproducible method follows the highly praised format of the Methods in Molecular Biologyâ„¢ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.

Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armamentarium of techniques in the urgent search for rapid and robust results

Editors and Affiliations

  • School of Life Sciences, University of Hertfordshire, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: The Protein Protocols Handbook

  • Editors: John M. Walker

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-59745-198-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-880-5Published: 07 October 2009

  • Softcover ISBN: 978-1-60327-474-6Published: 07 October 2009

  • eBook ISBN: 978-1-59745-198-7Published: 09 June 2010

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 3

  • Number of Pages: LXX, 1984

  • Topics: Protein Science, Posttranslational Modification

Buy it now

Buying options

Softcover Book USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access