Table of contents

  1. Front Matter
    Pages i-xxxv
  2. Quantitation of Proteins

    1. Alastair Aitken, Michèle P. Learmonth
      Pages 3-6
    2. Jakob H. Waterborg
      Pages 7-10
    3. Nicholas J. Kruger
      Pages 17-24
    4. Robert E. Akins, Rocky S. Tuan
      Pages 25-34
    5. Scott A. Boerner, Crescent R. Isham, Yean Kit Lee, Jennifer D. Tibodeau, Scott H. Kaufmann, Keith C. Bible
      Pages 35-45
    6. Alastair Aitken, Michèle P. Learmonth
      Pages 47-50
    7. Douglas D. Root, Kuan Wang
      Pages 51-54
    8. Thomas D. Friedrich, F. Andrew Ray, Ralph L. Smith, John M. Lehman
      Pages 55-61
    9. Thomas D. Friedrich, Ralph L. Smith, John M. Lehman
      Pages 63-69
  3. Electrophoresis of Proteins and Peptides and Dectection in Gels

    1. Sebastien Christian Carpentier, Rony Swennen, Bart Panis
      Pages 109-119
    2. Brian B. Vandahl, Gunna Christiansen, Svend Birkelund
      Pages 121-130
    3. Anthony G. Sullivan, Henry Brzeski, Jeban Ganesalingam, Manuel Mayr
      Pages 131-138
    4. Lynn A. Echan, David W. Speicher
      Pages 139-153
    5. Joakim Norbeck
      Pages 155-158
    6. Jens Schindler, Hans Gerd Nothwang
      Pages 159-164
    7. Hans Gerd Nothwang, Isabelle Guillemin, Jens Schindler
      Pages 165-170

About this book

Introduction

Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry.  This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods.  Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function. 

Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances.  The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.

Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.

Keywords

Antibodies Electrophoresis Post-translational modifications Protein blotting Protein function Translation proteins

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.School of Life SciencesUniversity of HertfordshireHatfieldUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-198-7
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-474-6
  • Online ISBN 978-1-59745-198-7
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • About this book