High Throughput Protein Expression and Purification

Methods and Protocols

  • Sharon A. Doyle

Part of the Methods in Molecular Biology book series (MIMB, volume 498)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Stephen P. Chambers, Susanne E. Swalley
    Pages 19-29
  3. Dominic Esposito, Leslie A. Garvey, Chacko S. Chakiath
    Pages 31-54
  4. Paul G. Blommel, Peter A. Martin, Kory D. Seder, Russell L. Wrobel, Brian G. Fox
    Pages 55-73
  5. Nick S. Berrow, David Alderton, Raymond J. Owens
    Pages 75-90
  6. William H. Eschenfeldt, Stols Lucy, Cynthia Sanville Millard, Andrzej Joachimiak, I. Donnelly Mark
    Pages 105-115
  7. James M. Abdullah, Andrzej Joachimiak, Frank R. Collart
    Pages 117-127
  8. Stephen P. Chambers, John R. Fulghum, Douglas A. Austen, Fan Lu, Susanne E. Swalley
    Pages 143-156
  9. Alison R. Gillies, Reza Banki Mahmoud, David W. Wood
    Pages 173-183
  10. Brian K. Kay, Sang Thai, Veronica V. Volgina
    Pages 185-198
  11. Mirjam Buchs, Ernie Kim, Yann Pouliquen, Michael Sachs, Sabine Geisse, Marion Mahnke et al.
    Pages 199-227
  12. Kalavathy Sitaraman, Deb K. Chatterjee
    Pages 229-244
  13. Joanne E. Nettleship, Nahid Rahman-Huq, Raymond J. Owens
    Pages 245-263
  14. Jenny A. Cappuccio, Angela K. Hinz, Edward A. Kuhn, Julia E. Fletcher, Erin S. Arroyo, Paul T. Henderson et al.
    Pages 273-295
  15. Joseph E. Tropea, Scott Cherry, David S. Waugh
    Pages 297-307
  16. Back Matter
    Pages 315-322

About this book


Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.


Cloning Escherichia coli Genome sequencing PCR Parallel processing membrane proteins molecular biology proteins

Editors and affiliations

  • Sharon A. Doyle
    • 1
  1. 1.Glaxo Smith KlineWalnut CreekUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-879-9
  • Online ISBN 978-1-59745-196-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site