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© 2008

SARS- and Other Coronaviruses

Laboratory Protocols

  • Dave Cavanagh

Part of the Methods in Molecular Biology book series (MIMB, volume 454)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Detection and Discovery of Coronaviruses

  3. Isolation, Growth, Titration, and Purification of Coronaviruses

  4. Structure of Covs Analyzed by Electron Microscopy

    1. Front Matter
      Pages 127-127
    2. Benjamin W. Neuman, Brian D. Adair, Mark Yeager, Michael J. Buchmeier
      Pages 129-136
  5. Expression of Coronavirus Proteins and Crystallization

    1. Front Matter
      Pages 137-137
    2. Yvonne Piotrowski, Rajesh Ponnusamy, Stephanie Glaser, Anniken Daabach, Ralf Moll, Rolf Hilgenfeld
      Pages 139-159
  6. Raising Antibodies to Coronavirus Proteins

    1. Front Matter
      Pages 161-161
    2. Amanda R. Pendleton, Carolyn E. Machamer
      Pages 163-189

About this book

Introduction

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.

Keywords

Antigen Coronavirus genomes Hepatitis PCR Severe acute respiratory syndrome coronavirus Vaccine development Viral protein Virus bacteria cell lines electron microscopy genes infectious transcription vaccine

Editors and affiliations

  • Dave Cavanagh
    • 1
  1. 1.Div. Molecular Biology, Compton LaboratoryInstitute Animal HealthNewbury, Berks.United Kingdom

Bibliographic information

  • Book Title SARS- and Other Coronaviruses
  • Book Subtitle Laboratory Protocols
  • Editors Dave Cavanagh
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI https://doi.org/10.1007/978-1-59745-181-9
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-867-6
  • Softcover ISBN 978-1-61737-815-7
  • eBook ISBN 978-1-59745-181-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XV, 326
  • Number of Illustrations 64 b/w illustrations, 0 illustrations in colour
  • Topics Medical Microbiology
    Infectious Diseases
    Virology
    Laboratory Medicine
  • Buy this book on publisher's site

Reviews

From the reviews:

"The serious nature of coronaviruses became well known with the discovery of the severe acute respiratory syndrome (SARS) virus which spread rapidly with life-threatening consequences in humans. ... this book describes the laboratory techniques used to study them. ... Scientists and students working in virology laboratories and, specifically, laboratories working with coronaviruses are the intended audience. ... each chapter contains a copious amount of detailed information as well as specific notes with technical advice. This is a valuable resource for scientists working with coronaviruses." (Rebecca T. Horvat, Doody's Review Service, November, 2008)

"This is volume 454 in the series Methods in Molecular Biology. … if you work on coronaviruses, or want to enter the field, this is a must-have book at an affordable price." (Stuart Siddell, Microbiology Today, February, 2009)