Exocytosis and Endocytosis

  • Andrei I. Ivanov

Part of the Methods in Molecular Biology book series (MIMB, volume 440)

Table of contents

  1. Front Matter
    Pages i-xv
  2. An Overview of Methods for Analyzing Vesicular Trafficking

  3. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis

  4. Analysis of Endocytosis and Exocytosis in Different Cell Systems

    1. Roland Govers, David E. James, Adelle C.F. Coster
      Pages 129-146
    2. Walter Stockinger, Axel Nohturfft
      Pages 147-155
    3. Asli Oztan, Christine Rondanino, Gerard Apodaca
      Pages 157-170
    4. Véronique Proux-Gillardeaux, Thierry Galli
      Pages 187-201
    5. Munekazu Yamakuchi, Marcella Ferlito, Craig N. Morrell, Kenji Matsushita, Craig A. Fletcher, Wangsen Cao et al.
      Pages 203-215
    6. Holger Lorenz, Dale W. Hailey, Jennifer Lippincott-Schwartz
      Pages 227-233
    7. Alan R. Burns, Janet M. Oliver, Janet R. Pfeiffer, Bridget S. Wilson
      Pages 235-245

About this book

Introduction

Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts provide the most up-to-date,step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. Following the highly successful Methods in Molecular Biology™ series format, the chapters present an introduction outlining the principle behind each technique, a list of the necessary materials, an easy to follow, readily reproducible protocol, and a Notes section offering tips on troubleshooting and avoiding known pitfalls.

Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.

Keywords

Cellular physiology Eukaryotic cells Intracellular processes Membrane-coated vesicles Plasma membrane Vivo biological functions cellular processes electron microscopy endothelium molecular biology

Editors and affiliations

  • Andrei I. Ivanov
    • 1
  1. 1.Department of Medicine Gastroenterology and Hepatology DivisionUniversity of Rochester School of Medicine and DentistryRochesterUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-178-9
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-865-2
  • Online ISBN 978-1-59745-178-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book