Immobilization of Enzymes and Cells

  • Jose M. Guisan

Part of the Methods in Biotechnology™ book series (MIBT, volume 22)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Beatriz M. Brena, Francisco Batista-Viera
    Pages 15-30
  3. Roger A. Sheldon, Rob Schoevaart, Luuk M. van Langen
    Pages 31-45
  4. Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan et al.
    Pages 47-55
  5. Lorena Betancor, Fernando López-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente et al.
    Pages 57-64
  6. Mónica Campás, Jean-Louis Marty
    Pages 77-85
  7. Ipsita Roy, Munishwar N. Gupta
    Pages 87-95
  8. Silvana Andreescu, Bogdan Bucur, Jean-Louis Marty
    Pages 97-106
  9. Ipsita Roy, Munishwar N. Gupta
    Pages 107-116
  10. Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan et al.
    Pages 117-128
  11. Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo et al.
    Pages 129-141
  12. Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente et al.
    Pages 143-152
  13. Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes et al.
    Pages 153-162
  14. Tamara Montes, Fernando López-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor et al.
    Pages 163-173
  15. Francisco Batista-Viera, Karen Ovsejevi, Carmen Manta
    Pages 185-204
  16. Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego et al.
    Pages 205-216
  17. Martina Koneracká, Peter Kopčanský, Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan et al.
    Pages 217-228

About this book


The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.


Activation Glucose enzyme enzymes protein

Editors and affiliations

  • Jose M. Guisan
    • 1
  1. 1.Institute of Catalysis, CSICCampus UAM-CantoblancoMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-290-2
  • Online ISBN 978-1-59745-053-9
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • About this book