Molecular Biomethods Handbook

1. Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes

   • M. Alexandra Aitken, Selina Raguz, Michael Antoniou

   Pages 235-250

2. Plant Transformation

   • Andy Prescott, Rob Briddon, Wendy Harwood

   Pages 251-269

3. Restriction Fragment-Length Polymorphisms

   • Elaine K. Green

   Pages 271-279

4. Genome Mapping

   • Jacqueline Boultwood

   Pages 281-285

5. Yeast Artificial Chromosomes

   • Angela Flannery, Rakesh Anand

   Pages 287-303

6. Polymerase Chain Reaction

   • Ralph Rapley

   Pages 305-325

7. In Vitro Transcriptions

   • Martin J. Tymms

   Pages 327-334

8. In Vitro Translation

   • Martin J. Tymms

   Pages 335-346

9. Site-Directed Mutagenesis

   • John R. Adair, T. Paul Wallace

   Pages 347-360

10. Site-Directed Mutagenesis

    • Roberta M. James, Paul Dickinson

    Pages 361-381

11. DNA Profiling

    • Karen M. Sullivan

    Pages 383-400

12. Radiolabeling of Peptides and Proteins

    • Arvind C. Patel, Stewart R. Matthewson

    Pages 411-411

13. Protein Electrophoresis

    • Paul Richards

    Pages 413-424

14. Free Zone Capillary Electrophoresis

    • David J. Begley

    Pages 425-434

15. Protein Blotting

    • Peter R. Shewry, Roger J. Fido

    Pages 435-444

16. Ion-Exchange Chromatography

    • David Sheehan, Richard Fitzgerald

    Pages 445-449

17. Size-Exclusion Chromatography

    • Paul Cutler

    Pages 451-460

18. Hydrophobic Interaction Chromatography

    • Paul A. O’Farrell

    Pages 461-467

19. Affinity Chromatography

    • George W. Jack

    Pages 469-477

20. Reversed-Phase HPLC

    • Bill Neville

    Pages 479-489

In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.

The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully.The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.

• antibody
• chromosome
• DNA
• enzyme
• flow cytometry
• gene
• gene transfer
• genome
• glycoprotein
• monoclonal antibodies
• RNA
• tissue
• transcription

"A good book for those who have been 'brought up' on kits and for those who would like to understand a little bit more about how a technique works rather than simply use it."-Microbiology Today

". . .a good lab investment."-Trends in Genetics

". . .a good teaching resource for undergraduates learning modern molecular genetic techniques. . .provides a clear introduction to many molecular biology techniques. . .best suited to mouse and human genetics."-Molecular Biotechnology

• University of Herfordshire, UK

  Ralph Rapley, John M. Walker

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