Downstream Processing of Proteins

Methods and Protocols

  • Mohamed A. Desai

Part of the Methods in Biotechnology book series (MIBT, volume 9)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Andrew L. Zydney, Ralf Kuriyel
    Pages 23-34
  3. Mohamed A. Desai, Mark Rayner, Mike Burns, Dervilla Bermingham
    Pages 73-94
  4. Ken Jones, Dev Baines
    Pages 95-107
  5. Allan Lihme, Marie Hansen, Morten Olander, Elias Zafirakos
    Pages 121-139
  6. Paul Bradley, Mohamed A. Desai
    Pages 141-160
  7. Yoichiro Shibusawa, Kazuhiro Matsuda, Yoichiro Ito
    Pages 161-172
  8. Ralf Kuriyel, Andrew L. Zydney
    Pages 185-194
  9. Back Matter
    Pages 223-229

About this book


In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process.
Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.

Editors and affiliations

  • Mohamed A. Desai
    • 1
  1. 1.Medeva Pharma Ltd.LiverpoolUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-564-5
  • Online ISBN 978-1-59259-027-8
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site