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© 2020

Bioluminescent Imaging

Methods and Protocols

  • Steven Ripp
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2081)

Table of contents

  1. Front Matter
    Pages i-xi
    PDF

  2. In Vitro/Ex Vivo Imaging Applications

    1. Front Matter
      Pages 1-1
      PDF
    2. High-Throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines
      Maria Maddalena Calabretta, Laura Montali, Antonia Lopreside, Elisa Michelini, Aldo Roda
      Pages 3-14
      PDF
    3. Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells
      Setsuko Tsuboi, Takashi Jin
      Pages 15-27
      PDF
    4. High-Throughput Analysis of Endocrine-Disrupting Compounds Using BLYES and BLYAS Bioluminescent Yeast Bioassays
      Tingting Xu, Anna Young, Jasleen Narula, Gary Sayler, Steven Ripp
      Pages 29-41
    5. Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon
      Carola Gregor
      Pages 43-52
      PDF
    6. Bioluminescent Imaging and Tracking of Bacterial Transport in Soils
      Jie Zhuang, Weipeng Liu, Liqiong Yang, Jia Kang, Xiaoming Zhang
      Pages 53-65
      PDF

  3. In Vivo Imaging of Microbial Pathogenesis

    1. Front Matter
      Pages 67-67
      PDF
    2. In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish
      José A. Guijarro, Jessica Méndez
      Pages 69-80
      PDF
    3. In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery
      Diana Caridha, Susan Leed, Alicia Cawlfield
      Pages 81-106
    4. Multiplex Imaging of Polymicrobial Communities—Murine Models to Study Oral Microbiome Interactions
      Jens Kreth, Yasser M. Abdelrahman, Justin Merritt
      Pages 107-126
    5. Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity In Vitro and In Vivo
      Katrien Van Dyck, Patrick Van Dijck, Greetje Vande Velde
      Pages 127-143

  4. In Vivo Small Animal/Organism Imaging Applications

    1. Front Matter
      Pages 145-145
      PDF
    2. Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson’s Disease
      Maria Eugenia Bernis, Gültekin Tamgüney
      Pages 147-159
      PDF
    3. Continual Conscious Bioluminescent Imaging in Freely Moving Mice
      Juan Antinao Diaz, Amy Geard, Lorna M. FitzPatrick, Juliette M. K. M. Delhove, Suzanne M. K. Buckley, Simon N. Waddington et al.
      Pages 161-175
      PDF
    4. In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent Model
      Shan Lei, Weijin Huang, Youchun Wang, Qiang Liu
      Pages 177-190
    5. Continuous and Real-Time In Vivo Autobioluminescent Imaging in a Mouse Model
      Derek Yip, Andrew Kirkpatrick, Tingting Xu, Tom Masi, Stacy Stephenson, Steven Ripp et al.
      Pages 191-201
      PDF
    6. In Vivo Tracking of Tumor-Derived Bioluminescent Extracellular Vesicles in Mice
      Prakash Gangadaran, Byeong-Cheol Ahn
      Pages 203-210
    7. Bioluminescence Imaging in the Chick Chorioallantoic Membrane Assay
      Benedict Jefferies, Zhichao Tong, Roman Nawroth
      Pages 211-217
      PDF
    8. Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models
      Xiang Li, Minyong Li
      Pages 219-228
      PDF

  5. In Planta Imaging Applications

    1. Front Matter
      Pages 229-229
      PDF
    2. Application of Single-Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant Cells
      Tomoaki Muranaka, Tokitaka Oyama
      Pages 231-242
      PDF
  6. Back Matter
    Pages 243-246
    PDF

About this book

Introduction

This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

Keywords

luciferases FUEL ALLIGATOR bacterial trafficking Caenorhabditis elegans

Editors and affiliations

  • Steven Ripp
    • 1
  1. 1.The Center for Environmental BiotechnologyThe University of Tennessee, 490 BioTech Inc.KnoxvilleUSA

About the editors

.

Bibliographic information

  • Book Title Bioluminescent Imaging
  • Book Subtitle Methods and Protocols
  • Editors Steven Ripp
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI https://doi.org/10.1007/978-1-4939-9940-8
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9939-2
  • Softcover ISBN 978-1-4939-9942-2
  • eBook ISBN 978-1-4939-9940-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 246
  • Number of Illustrations 21 b/w illustrations, 63 illustrations in colour
  • Topics Imaging / Radiology
    Biotechnology
  • Buy this book on publisher's site

Reviews

“The book is intended for students and researchers who are interested in learning more about bioluminescent imaging. … This book's unique feature is that it provides cutting-edge, detailed protocols and methodologies required to perform bioluminescent imaging, which has widespread applications to address basic research questions in cellular and molecular biology.” (Omer Iqbal, Doody's Book Reviews, October 16, 2020)