Transgenic Mouse

Methods and Protocols

  • Melissa A. Larson

Part of the Methods in Molecular Biology book series (MIMB, volume 2066)

Table of contents

  1. Front Matter
    Pages i-x
  2. Thomas L. Saunders
    Pages 1-26
  3. Melissa A. Larson
    Pages 27-33
  4. Melissa A. Larson, Katelin A. Gibson, Jay L. Vivian
    Pages 47-57
  5. Marina Gertsenstein, Joffrey Mianné, Lydia Teboul, Lauryl M. J. Nutter
    Pages 59-82
  6. Melissa A. Larson
    Pages 101-106
  7. Ling Liu
    Pages 113-124
  8. Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, Roberto Giovannoni
    Pages 125-132
  9. Neeraj K. Aryal, Jan Parker-Thornburg
    Pages 133-148
  10. Alicia Valenzuela
    Pages 149-162
  11. Jan Parker-Thornburg
    Pages 163-169
  12. Toru Takeo, Naomi Nakagata
    Pages 195-209
  13. Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata
    Pages 211-216
  14. Back Matter
    Pages 235-236

About this book


This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. 


pronuclear injection embryonic stem cells Enzymatic modifier systems Spermatogonial Nuclear transfer

Editors and affiliations

  • Melissa A. Larson
    • 1
  1. 1.Department of Molecular and Integrative Physiology, University of Kansas Medical CenterTransgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USAKansas CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9836-4
  • Online ISBN 978-1-4939-9837-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site