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Electroporation Protocols

Microorganism, Mammalian System, and Nanodevice

  • Shulin Li
  • Lingqian Chang
  • Justin Teissie
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2050)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Design and Fabrication of Micro-/Nano-Electroporation

    1. Front Matter
      Pages 1-1
    2. Xuan Liu, Yingbo Zu, Shengnian Wang
      Pages 3-12
    3. Lingqian Chang, Stephen Black, Chandani Chitrakar, Mehdi Nouri
      Pages 29-41
    4. Juliette Experton, Aaron G. Wilson, Charles R. Martin
      Pages 43-55
  3. Micro-/Nano-Electroporation for In Vitro Delivery

    1. Front Matter
      Pages 57-57
    2. Horacio D. Espinosa, Prithvijit Mukherjee, Cesar Patino
      Pages 59-68
    3. Lingqian Chang, Chandani Chitrakar, Mehdi Nouri
      Pages 69-77
    4. Silvia Duarte-Sanmiguel, Natalia Higuita-Castro, Daniel Gallego-Perez
      Pages 79-84
    5. Justin Brooks, Arian Jaberi, Ruiguo Yang
      Pages 91-97
  4. Advanced In Vivo Electroporation

    1. Front Matter
      Pages 99-99
    2. Dong Huang, Yuanyu Huang, Zhihong Li
      Pages 101-112
    3. Daniel Schwarz, Andreas T. Schaefer
      Pages 113-120
    4. Diego Alzate-Correa, William Lawrence, Natalia Higuita-Castro, Daniel Gallego-Perez
      Pages 145-152
  5. Microorganism Electroporation

    1. Front Matter
      Pages 153-153
    2. Da-Wei Li, Srinivasan Balamurugan, Jian-Wei Zheng, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li
      Pages 175-179
    3. E. Meilhoc, J. Teissie
      Pages 187-193
  6. Back Matter
    Pages 195-196

About this book

Introduction

This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field. 

Keywords

dose control single cell transfection Array Electroporation neuronal network mouse zygotes CRISPR/Cas9

Editors and affiliations

  • Shulin Li
    • 1
  • Lingqian Chang
    • 2
  • Justin Teissie
    • 3
  1. 1.MD Anderson Cancer CenterThe University of TexasHoustonUSA
  2. 2.School of Biological Science and Medical Engineering, Beihang University, Beijing, ChinaInstitute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,BeijingChina
  3. 3.Institut de Pharmacologie et de Biologie StructuraleUniversité de Toulouse, CNRS, UPSToulouseFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9740-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9739-8
  • Online ISBN 978-1-4939-9740-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site