In Vitro Differentiation of T-Cells

Methods and Protocols

  • Shin Kaneko

Part of the Methods in Molecular Biology book series (MIMB, volume 2048)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jacqueline Flynn, Paul Gorry
    Pages 1-13
  3. Jacqueline Flynn, Paul Gorry
    Pages 15-25
  4. Taisuke Kondo, Yuki Imura, Makoto Ando, Shunsuke Chikuma, Akihiko Yoshimura
    Pages 41-51
  5. Toshinobu Nishimura, Yukiko Murmann, Hiromitsu Nakauchi
    Pages 53-57
  6. Yutaka Yasui, Yasumichi Hitoshi, Shin Kaneko
    Pages 77-80
  7. Tatsuki Ueda, Shin Kaneko
    Pages 85-91
  8. Rong Zhang, Shuichi Kitayama, Tianyi Liu, Norihiro Ueda, Yumi Tokumitsu, Hiroaki Mashima et al.
    Pages 121-130
  9. Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, Jianxun Song
    Pages 131-141
  10. Miho Sekai, Jianwei Wang, Yoko Hamazaki
    Pages 143-153
  11. Willem Van Der Byl, Simone Rizzetto, Jerome Samir, Curtis Cai, Auda A. Eltahla, Fabio Luciani
    Pages 155-205
  12. Songling Li, Jan Wilamowski, Shunsuke Teraguchi, Floris J. van Eerden, John Rozewicki, Ana Davila et al.
    Pages 207-229
  13. Tinhinane Fali, Camille K’Ros, Victor Appay, Delphine Sauce
    Pages 231-243
  14. Jonathan Villanueva, Toshinobu Nishimura, Hiromitsu Nakauchi
    Pages 259-264
  15. Back Matter
    Pages 265-267

About this book


This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.


Pluripotent stem cells Human embryonic stem cells iPSC generation Cellular medicines Human genetics

Editors and affiliations

  • Shin Kaneko
    • 1
  1. 1.Shin Kaneko Laboratory, Department of Cell Growth and DifferentiationCenter for iPS Cell Research and Application, Kyoto UniversityKyotoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9727-5
  • Online ISBN 978-1-4939-9728-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site