© 2019

Computer Optimized Microscopy

Methods and Protocols

  • Elena Rebollo
  • Manel Bosch

Part of the Methods in Molecular Biology book series (MIMB, volume 2040)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Overview

    1. Front Matter
      Pages 1-1
    2. José F. Pertusa, Jose M. Morante-Redolat
      Pages 3-21
    3. Romain Guiet, Olivier Burri, Arne Seitz
      Pages 23-37
  3. Methods Based on ImageJ Macro Programming

    1. Front Matter
      Pages 39-39
    2. Marc López-Cano, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 41-50
    3. Bertrand Cinquin, Filipa Lopes
      Pages 117-133
    4. Hanh Nguyen, Jaume Boix-Fabrés, Nadine Peyriéras, Elena Kardash
      Pages 135-153
    5. Jaume Boix-Fabrés, Katerina Karkali, Enrique Martín-Blanco, Elena Rebollo
      Pages 155-175
    6. Patrice Mascalchi, Fabrice P. Cordelières
      Pages 177-213
    7. Daniel Sastre, Irene Estadella, Manel Bosch, Antonio Felipe
      Pages 215-233
    8. Pau Carrillo-Barberà, Jose M. Morante-Redolat, José F. Pertusa
      Pages 299-329
    9. Joaquim Soriano, Gadea Mata, Diego Megias
      Pages 331-356
  4. Methods Based on ImageJ Plugin Development

    1. Front Matter
      Pages 357-357
    2. Guillaume Jacquemet, Hellyeh Hamidi, Johanna Ivaska
      Pages 359-373

About this book


This volume explores open-source based image analysis techniques to provide a state-of-the-art collection of workflows covering current bioimage analysis problematics, including colocalization, particle counting, 3D structural analysis, ratio imaging and FRET quantification, particle tracking, high-content screening or machine learning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and scripts, step-by-step, readily reproducible image analysis protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Computer Optimized Microscopy: Methods and Protocols is a valuable resource for both novice and experienced researchers who are interested in learning more about this developing field. 


pixel‐intensity‐correlation. single particle tracking kymograph analysis. Proximity Ligation Assay digital image quantification FRET quantification 3D structural analysis

Editors and affiliations

  • Elena Rebollo
    • 1
  • Manel Bosch
    • 2
  1. 1.Molecular Imaging PlatformMolecular Biology Institute of Barcelona (CSIC)BarcelonaSpain
  2. 2.Scientific and Technological Centers (CCiTUB)Universitat de BarcelonaBarcelonaSpain

Bibliographic information

  • Book Title Computer Optimized Microscopy
  • Book Subtitle Methods and Protocols
  • Editors Elena Rebollo
    Manel Bosch
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-9685-8
  • Softcover ISBN 978-1-4939-9688-9
  • eBook ISBN 978-1-4939-9686-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 467
  • Number of Illustrations 8 b/w illustrations, 181 illustrations in colour
  • Topics Imaging / Radiology
    Biological Microscopy
  • Buy this book on publisher's site