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Imaging Gene Expression

Methods and Protocols

  • Yaron Shav-Tal
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2038)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Imaging RNAs and Gene Expression in Cells and Organisms

    1. Front Matter
      Pages 1-1
    2. Maria Vera, Evelina Tutucci, Robert H. Singer
      Pages 3-20
    3. Yachen Ying, Shiqi Mao, Christopher J. Krueger, Antony K. Chen
      Pages 21-33
    4. Jae Youn Shim, Byung Hun Lee, Hye Yoon Park
      Pages 47-61
    5. Carola Fernandez, Mounia Lagha
      Pages 63-74
    6. Franka Voigt, Jan Eglinger, Jeffrey A. Chao
      Pages 75-88
    7. Kristina A. Ganzinger, Martin R. Challand, James Spencer, David Klenerman, Rohan T. Ranasinghe
      Pages 89-107
    8. Azra Lari, Farzin Farzam, Pierre Bensidoun, Marlene Oeffinger, Daniel Zenklusen, David Grunwald et al.
      Pages 131-150
  3. Imaging Chromosomes, Genes, and Transcription Factors

    1. Front Matter
      Pages 165-165
    2. Son C. Nguyen, Eric F. Joyce
      Pages 167-180
    3. Kathrin S. Heinz, M. Cristina Cardoso
      Pages 199-208
    4. Sascha Conic, Dominique Desplancq, Alexia Ferrand, Nacho Molina, Etienne Weiss, László Tora
      Pages 209-221
    5. Jorge Trojanowski, Anne Rademacher, Fabian Erdel, Karsten Rippe
      Pages 251-270
  4. Back Matter
    Pages 271-272

About this book

Introduction

This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging. These updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, and factors that bind DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Imaging Gene Expression: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this evolving and important field. 

Keywords

FISH Drosophila mRNAs Endoplasmic Reticulum. RNA Pol II CRISPR

Editors and affiliations

  • Yaron Shav-Tal
    • 1
  1. 1.The Mina & Everard Goodman, Faculty of Life Sciences and the Institute of Nanotechnology and Advanced MaterialsBar-Ilan UniversityRamat GanIsrael

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9674-2
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9673-5
  • Online ISBN 978-1-4939-9674-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site