Oligonucleotide-Based Therapies

Methods and Protocols

  • Olof Gissberg
  • Rula Zain
  • Karin E. Lundin

Part of the Methods in Molecular Biology book series (MIMB, volume 2036)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Karin E. Lundin, Olof Gissberg, C. I. Edvard Smith, Rula Zain
      Pages 3-16
    3. Xin Xia, Nicolette Pollock, Jiehua Zhou, John Rossi
      Pages 17-50
  3. Protocols and Strategies

    1. Front Matter
      Pages 51-51
    2. Linda Vidarsdottir, Oksana Goroshchuk, Iryna Kolosenko, Caroline Palm-Apergi
      Pages 53-72
    3. Negin Mozafari, Tea Umek
      Pages 91-112
    4. Lennart Nilsson, Alessandra Villa
      Pages 113-126
    5. Philipp Michael Klein, Ernst Wagner
      Pages 141-164
    6. Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos
      Pages 187-203 Open Access
    7. Pedro M. D. Moreno, Teresa Rodrigues, Marília Torrado, Isabel F. Amaral, Ana P. Pêgo
      Pages 205-219
    8. Suzan M. Hammond, Frank Abendroth, Michael J. Gait, Matthew J. A. Wood
      Pages 221-236
    9. Sabine Sewing, Tanja Minz, Franziska Boess
      Pages 249-259
    10. Christophe Husser, Erich Koller, Andreas Brink, Simone Schadt
      Pages 307-315
    11. Rasmus Jansson-Löfmark, Christine Ahlström, Peter Gennemark
      Pages 317-339
  4. Back Matter
    Pages 341-344

About this book


This book provides a collection of comprehensive, up-to-date, and broadly applicable guides to the research and development fields of oligonucleotide (ON) therapeutics. Covering topics from the study of antisense and anti-gene effects to oligonucleotides in the context of drug discovery and development, the volume explores a wide-ranging and useful spectrum of methods and protocols needed to take full advantage of therapeutic applications involving ONs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Oligonucleotide-Based Therapies: Methods and Protocols aims to be a great aid in the laboratory as well as an ideal reference guide when designing antisense and anti-gene oligonucleotides for therapeutic applications.


Drug discovery and development Targeting strategies Antisense Therapeutic applications Anti-gene oligonucleotides Aptamers

Editors and affiliations

  • Olof Gissberg
    • 1
  • Rula Zain
    • 2
  • Karin E. Lundin
    • 3
  1. 1.Center for Advanced Therapies, Department of Laboratory MedicineKarolinska InstitutetStockholmSweden
  2. 2.Center for Advanced Therapies, Department of Laboratory MedicineKarolinska InstitutetStockholmSweden
  3. 3.Center for Advanced Therapies, Department of Laboratory MedicineKarolinska InstitutetStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9669-8
  • Online ISBN 978-1-4939-9670-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site