Methods and Protocols

  • Olga Garaschuk
  • Alexei Verkhratsky

Part of the Methods in Molecular Biology book series (MIMB, volume 2034)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Microglia in Health and Disease

    1. Front Matter
      Pages 1-1
    2. Olga Garaschuk, Alexei Verkhratsky
      Pages 3-11
    3. Julie C. Savage, Micaël Carrier, Marie-Ève Tremblay
      Pages 13-26
    4. Olga Garaschuk, Alexei Verkhratsky
      Pages 27-40
    5. Hélène Hirbec, François Rassendren, Etienne Audinat
      Pages 41-55
    6. Darío Tejera, Michael T. Heneka
      Pages 57-67
    7. Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, Junichi Nabekura
      Pages 69-80
  3. In Vitro Analyses of Microglia

    1. Front Matter
      Pages 81-81
    2. Marie-Ève Tremblay, Ania K. Majewska
      Pages 83-95
    3. Marie-Kim St-Pierre, Maude Bordeleau, Marie-Ève Tremblay
      Pages 97-110
    4. Elena Avignone, Giampaolo Milior, Isabelle Arnoux, Etienne Audinat
      Pages 111-125
    5. Katherine Figarella, Nestor L. Uzcategui, Michael Duszenko
      Pages 149-161
  4. Studying Microglia In Vivo

    1. Front Matter
      Pages 163-163
    2. Bianca Brawek, Maria Olmedillas del Moral, Olga Garaschuk
      Pages 165-175
    3. Angelos A. Skodras, Jasmin K. Hefendehl, Jonas J. Neher
      Pages 177-189
    4. Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, Christian Madry
      Pages 191-205
    5. Diego Gomez-Nicola, Gemma L. Fryatt, Katharine E. Askew
      Pages 207-215
    6. Yajie Liang, Olga Garaschuk
      Pages 243-265
    7. Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, M. Yashar S. Kalani
      Pages 267-279
    8. Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, Hiroaki Wake
      Pages 281-286
    9. Darío Tejera, Michael T. Heneka
      Pages 287-292
    10. Nicholas H. Varvel, Richard M. Ransohoff, Jonas J. Neher
      Pages 293-301
  5. Omics Analyses of Microglia

    1. Front Matter
      Pages 303-303
    2. Suzanne E. Hickman, Joseph El Khoury
      Pages 305-323
    3. Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, Mélanie Morin-Brureau
      Pages 325-336
  6. Back Matter
    Pages 337-340

About this book


This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.


Functional brain networks Immune mediators Microglial research Synaptic contacts Cognitive ability Brain immunity

Editors and affiliations

  • Olga Garaschuk
    • 1
  • Alexei Verkhratsky
    • 2
  1. 1.Department of NeurophysiologyInstitute of Physiology, Eberhard Karls University of TübingenTübingenGermany
  2. 2.Faculty of Biology, Medicine and Health The University of Manchester, Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, SpainManchesterUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9657-5
  • Online ISBN 978-1-4939-9658-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site