Methods and Protocols

  • J. Philip McCoy, Jr

Part of the Methods in Molecular Biology book series (MIMB, volume 2032)

Table of contents

  1. Front Matter
    Pages i-x
  2. Gregory K. Behbehani
    Pages 31-51
  3. Valentina Giudice, Giovanna Fantoni, Angélique Biancotto
    Pages 53-68
  4. Janet Staats, Anagha Divekar, J. Philip McCoy Jr., Holden T. Maecker
    Pages 81-104
  5. Chen Ziqing, Andreas Lundqvist, Kristina Witt
    Pages 105-114
  6. Alain Diaz, Maria Romero, Daniela Frasca, Bonnie B. Blomberg
    Pages 115-127
  7. Ankit Saxena, Pradeep K. Dagur, Angélique Biancotto
    Pages 129-140
  8. Sara Trabanelli, Alejandra Gomez-Cadena, Camilla Jandus
    Pages 179-192
  9. Abdullah Alsuwaidan, Franklin Fuda, Weina Chen, Mingyi Chen
    Pages 193-201
  10. Moen Sen, Ling Wang, Liping Yu, Erica L. Carpenter
    Pages 213-226
  11. Bruce Greig
    Pages 227-279
  12. Pallavi Kanwar Galera, Chunjie Jiang, Raul Braylan
    Pages 281-296
  13. Joseph A. DiGiuseppe, Jolene L. Cardinali
    Pages 297-310
  14. Dalia A. Salem, Maryalice Stetler-Stevenson
    Pages 311-321
  15. Andrea J. Illingworth, Iuri Marinov, D. Robert Sutherland
    Pages 323-354
  16. Back Matter
    Pages 355-356

About this book


This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field. 


fluorescence-based cytometers lyophilized reagents mass cytometry B lymphocytes intracellular cytokines T regulatory cells lymphoid cells human stem cells leukemias

Editors and affiliations

  • J. Philip McCoy, Jr
    • 1
  1. 1.FrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9649-0
  • Online ISBN 978-1-4939-9650-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site