High-Throughput Protein Production and Purification

Methods and Protocols

  • Renaud Vincentelli

Part of the Methods in Molecular Biology book series (MIMB, volume 2025)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. General Procedures for High-Throughput Protein Production

    1. Front Matter
      Pages 1-1
    2. Claudia Ortega, Cecilia Abreu, Pablo Oppezzo, Agustín Correa
      Pages 3-32
    3. Joanne E. Nettleship, Heather Rada, Raymond J. Owens
      Pages 33-49
    4. Edward Kraft, Yvonne Franke, Katharine Heeringa, Stephanie Shriver, Inna Zilberleyb, Christine Kugel et al.
      Pages 51-68
    5. Francisco J. Fernández, Sara Gómez, M. Cristina Vega
      Pages 69-91
    6. Sarah M. Rue, Paul W. Anderson, Michelle R. Gaylord, Jessica J. Miller, Scott M. Glaser, Scott A. Lesley
      Pages 93-142
    7. Kenneth W. Walker, Philip An, Dwight Winters
      Pages 143-161
  3. High-Throughput Protocols Adapted to the Production of Specific Protein Families

    1. Front Matter
      Pages 163-163
    2. Yoan Duhoo, Ana Filipa Sequeira, Natalie J. Saez, Jeremy Turchetto, Laurie Ramond, Fanny Peysson et al.
      Pages 165-190
    3. Sebastian Mathea, Eidarus Salah, Stefan Knapp
      Pages 191-202
    4. Giulio Russo, Viola Fühner, André Frenzel, Michael Hust, Stefan Dübel
      Pages 203-211
    5. Duygu Sari-Ak, Shervin Bahrami, Magdalena J. Laska, Petra Drncova, Daniel J. Fitzgerald, Christiane Schaffitzel et al.
      Pages 213-226
    6. Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine
      Pages 261-279
    7. Claire Strain-Damerell, Nicola A. Burgess-Brown
      Pages 281-296
  4. High-Throughput Protein Production Combined with High Throughput Micro-Characterization for Protein Stability, Solubility, Quality and Interactions

    1. Front Matter
      Pages 297-297
    2. Amélie Massemin, Stéphanie Cabantous, Geoffrey S. Waldo, Jean-Denis Pedelacq
      Pages 321-333
    3. Hui Xu, Thomas Clairfeuille, Christine C. Jao, Hoangdung Ho, Zachary Sweeney, Jian Payandeh et al.
      Pages 389-402
    4. Wayne A. Johnston, Shayli Varasteh Moradi, Kirill Alexandrov
      Pages 403-421
    5. Jean-Denis Pedelacq, Geoffrey S. Waldo, Stéphanie Cabantous
      Pages 423-437
    6. Yoan Duhoo, Virginie Girault, Jeremy Turchetto, Laurie Ramond, Fabien Durbesson, Patrick Fourquet et al.
      Pages 439-476
    7. Kazuhiro R. Nitta, Renaud Vincentelli, Edwin Jacox, Agnès Cimino, Yukio Ohtsuka, Daniel Sobral et al.
      Pages 487-517
    8. Sara Gómez, Francisco J. Fernández, M. Cristina Vega
      Pages 519-531
  5. Back Matter
    Pages 533-537

About this book


This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.


Protein expression Micro-characterization Up-scaling production Soluble yield Structural genomics

Editors and affiliations

  • Renaud Vincentelli
    • 1
  1. 1.Architecture et Fonction des Macromolécules Biologiques (AFMB), Unité Mixte de Recherche (UMR) 7257Centre National de la Recherche Scientifique (CNRS), Aix-Marseille UniversitéMarseille cedex 9France

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9623-0
  • Online ISBN 978-1-4939-9624-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site