Methods and Protocols

  • Kelly M. Fulton
  • Susan M. Twine

Part of the Methods in Molecular Biology book series (MIMB, volume 2024)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, Felicity Stark
    Pages 1-24
  3. Kelly M. Fulton, Isabel Baltat, Susan M. Twine
    Pages 25-58
  4. Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, Susan M. Twine
    Pages 59-78
  5. Sophie Moggridge, Kelly M. Fulton, Susan M. Twine
    Pages 103-117
  6. N. Helena Beyer, Niels H. H. Heegaard
    Pages 119-135
  7. Christie E. Delaney, John F. Kelly, Wen Ding, Arsalan S. Haqqani
    Pages 153-166
  8. Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall
    Pages 199-211
  9. Rui Chen, Jianjun Li
    Pages 245-257
  10. Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, Shashi Gujar
    Pages 301-307
  11. Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler et al.
    Pages 327-332
  12. Nozomi Aibara, Kaname Ohyama
    Pages 333-338
  13. Richard Ouedraogo, Julien Textoris, Laurent Gorvel, Aurélie Daumas, Christian Capo, Jean-Louis Mege
    Pages 339-351
  14. Back Matter
    Pages 379-380

About this book


This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field. 


MHC peptides Immunogenicity autoimmunity Glycopeptides SERPA

Editors and affiliations

  • Kelly M. Fulton
    • 1
  • Susan M. Twine
    • 2
  1. 1.Human Health Therapeutics Research CentreNational Research Council of CanadaOttawaCanada
  2. 2.Human Health Therapeutics Research CentreNational Research Council of CanadaOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9596-7
  • Online ISBN 978-1-4939-9597-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site