Retinoid and Rexinoid Signaling

Methods and Protocols

  • Swapan K. Ray

Part of the Methods in Molecular Biology book series (MIMB, volume 2019)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Jie Liu, Jian-gang Huang, Jin-Zhang Zeng
    Pages 15-31
  3. Nina Orfali, Jennie N. Jeyapalan, Corinne L. Woodcock, Tracey R. O’Donovan, Dalyia Benjamin, Mary Cahill et al.
    Pages 33-49
  4. Claudio Martínez, José A. Souto, Angel R. de Lera
    Pages 51-72
  5. Haider M. Hassan, T. Michael Underhill, Joseph Torchia
    Pages 129-141
  6. M. Handberg-Thorsager, V. Ulman, P. Tomançak, D. Arendt, M. Schubert
    Pages 193-207
  7. Sarah Parejo, Mario P. Tschan, Manuele G. Muraro, Enrico Garattini, Giulio C. Spagnoli, Anna M. Schläfli
    Pages 237-256
  8. Back Matter
    Pages 275-276

About this book


This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and larval swimming behavior; and protocols for assessment of autophagic flux in ATRA treated 2D and 3D breast cancer cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Retinoid and Rexinoid Signaling: Methods and Protocols is a valuable resources for graduate students, postdoctoral fellows, and principal investigators who are interested in further exploring the signaling mechanisms of these molecules in their specific preclinical models.


retinoic acid receptor-gamma hepatocellular carcinoma of GSK3β/β-catenin signaling retinoid X Receptor (RXR)-selective agonists Huntington's disease

Editors and affiliations

  • Swapan K. Ray
    • 1
  1. 1.Department of Pathology, Microbiology, and ImmunologyUniversity of South Carolina School of MedicineColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9584-4
  • Online ISBN 978-1-4939-9585-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site