Citrus Tristeza Virus

Methods and Protocols

  • Antonino F. Catara
  • Moshe Bar-Joseph
  • Grazia Licciardello

Part of the Methods in Molecular Biology book series (MIMB, volume 2015)

Table of contents

  1. Front Matter
    Pages i-x
  2. Marcella Russo, Antonino F. Catara
    Pages 15-27
  3. Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, Francesco Di Serio
    Pages 67-78
  4. Elisavet K. Chatzivassiliou, Grazia Licciardello
    Pages 79-104
  5. Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, Raymond Yokomi
    Pages 105-126
  6. Munir Mawassi, Sabrina Haviv, Ludmila Maslenin
    Pages 151-161
  7. Ana Belén Ruiz-García, Rachelle Bester, Antonio Olmos, Hans Jacob Maree
    Pages 163-178
  8. Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio et al.
    Pages 195-207
  9. Milena Santos Dória, Carlos Priminho Pirovani
    Pages 209-218
  10. Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores et al.
    Pages 229-243
  11. Back Matter
    Pages 245-247

About this book


This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.


CTV Plant virologists Deep sequencing Closterovirus Closteroviridae RT-PCR Plant pathologists

Editors and affiliations

  • Antonino F. Catara
    • 1
  • Moshe Bar-Joseph
    • 2
  • Grazia Licciardello
    • 3
  1. 1.Department of Phytosanitary SciencesUniversity of Catania, Science and Technology Park of SicilyCataniaItaly
  2. 2.The S. Talkowski Laboratory, Department of Plant Pathology and Weed ResearchThe Volcani Center, Agricultural Research OrganizationBet DaganIsrael
  3. 3.Consiglio per la Ricerca in agricoltura e l’analisi dell’Economia Agraria, Centro di Olivicoltura, Frutticoltura e Agrumicoltura (CREA-OFA)Acireale (Catania)Italy

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9557-8
  • Online ISBN 978-1-4939-9558-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site