Protein Lipidation

Methods and Protocols

  • Maurine E. Linder

Part of the Methods in Molecular Biology book series (MIMB, volume 2009)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Detection of Lipid Modifications

    1. Front Matter
      Pages 1-1
    2. Charlotte H. Hurst, Dionne Turnbull, Piers A. Hemsley
      Pages 3-11
    3. Jennifer Greaves, Nicholas C. O. Tomkinson
      Pages 13-33
    4. Mina Ahmadi, Kiall Francis Suazo, Mark D. Distefano
      Pages 35-43
    5. Melanie Cheung See Kit, Brent R. Martin
      Pages 71-79
  3. Reversible Fatty Acylation

    1. Front Matter
      Pages 81-81
    2. Laurence Abrami, Robin A. Denhardt-Eriksson, Vassily Hatzimanikatis, F. Gisou van der Goot
      Pages 111-127
    3. Jun Young Hong, Ji Cao, Hening Lin
      Pages 129-136
  4. zDHHC S-Acyltransferases

    1. Front Matter
      Pages 149-149
    2. Heather McClafferty, Michael J. Shipston
      Pages 151-168
    3. David A. Mitchell, Laura C. Pendleton, Robert J. Deschenes
      Pages 169-177
    4. Martin Ian P. Malgapo, Maurine E. Linder
      Pages 179-189
  5. Online Resource

    1. Front Matter
      Pages 201-201
    2. Mathieu Blanc, Fabrice P. A. David, F. Gisou van der Goot
      Pages 203-214
  6. Fatty Acylation in the Lumen of the Secretory Pathway

    1. Front Matter
      Pages 215-215
    2. Michelle A. Sieburg, Elizabeth R. Cleverdon, James L. Hougland
      Pages 227-241
    3. James John Asciolla, Kalpana Rajanala, Marilyn D. Resh
      Pages 243-255
  7. Prenylation and Post-Prenylation Processing

    1. Front Matter
      Pages 257-257
    2. William Gillette, Peter Frank, Shelley Perkins, Matthew Drew, Carissa Grose, Dominic Esposito
      Pages 259-277
    3. Erh-Ting Hsu, Jeffrey S. Vervacke, Mark D. Distefano, Christine A. Hrycyna
      Pages 279-293
  8. Biochemistry of Protein Lipidation

    1. Front Matter
      Pages 295-295
    2. Yang Gao, Jon W. Erickson, Richard A. Cerione, Sekar Ramachandran
      Pages 307-315
    3. Yang Gao, Jon W. Erickson, Richard A. Cerione, Sekar Ramachandran
      Pages 317-324
  9. Back Matter
    Pages 325-327

About this book


This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.


fatty acylation lipid modifications cysteine modifications Mass Spectrometry enzyme

Editors and affiliations

  • Maurine E. Linder
    • 1
  1. 1.Department of Molecular Medicine, College of Veterinary MedicineCornell UniversityIthacaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9531-8
  • Online ISBN 978-1-4939-9532-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site