Chimera Research

Methods and Protocols

  • Insoo Hyun
  • Alejandro De Los Angeles

Part of the Methods in Molecular Biology book series (MIMB, volume 2005)

Table of contents

  1. Front Matter
    Pages i-x
  2. Human Donor Cell Types: Potency and Position

  3. Non-Human Hosts: Species and Developmental Stages

    1. Front Matter
      Pages 75-75
    2. Iain Martyn, Tatiane Y. Kanno, Ali H. Brivanlou
      Pages 77-89
    3. Yoon-Sang Kim, Matthew Wielgosz, Byoung Ryu
      Pages 91-100
    4. Cuiqing Zhong, Jun Wu, Juan Carlos Izpisua Belmonte
      Pages 101-124
    5. Alejandro De Los Angeles, Masahiro Sakurai, Jun Wu
      Pages 125-151
    6. Alejandro De Los Angeles
      Pages 153-163
  4. New Directions and Controversies

    1. Front Matter
      Pages 173-173
    2. Sebastian Porsdam Mann, Rosa Sun, Göran Hermerén
      Pages 175-193
    3. Daniel Counihan
      Pages 195-203
    4. Nilam Sinha, Eoin C. Whelan, Ralph L. Brinster
      Pages 205-220
    5. Alejandro De Los Angeles, Insoo Hyun, Stephen R. Latham, John D. Elsworth, D. Eugene Redmond Jr.
      Pages 221-231
  5. Back Matter
    Pages 233-234

About this book


This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naïve and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes. 


Pluripotent Stem Cells Somatic Cells Whole-organ Transplantation Xenobarrier Parabiosis

Editors and affiliations

  • Insoo Hyun
    • 1
  • Alejandro De Los Angeles
    • 2
  1. 1.Department of BioethicsCase Western Reserve University, School of MedicineClevelandUSA
  2. 2.Department of PsychiatryYale University School of MedicineNew HavenUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9523-3
  • Online ISBN 978-1-4939-9524-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site