SMC Complexes

Methods and Protocols

  • Anjana Badrinarayanan

Part of the Methods in Molecular Biology book series (MIMB, volume 2004)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Depletion Systems to Assess SMC Function

  3. Genetic Manipulation of SMC Function

    1. Front Matter
      Pages 47-47
    2. Marie-Laure Diebold-Durand, Frank Bürmann, Stephan Gruber
      Pages 49-61
    3. Jan Josef Paleček, Lucie Vondrová, Kateřina Zábrady, Jakub Otočka
      Pages 79-90
    4. Paul Kalitsis, Tao Zhang, Ji Hun Kim, Christian F. Nielsen, Kathryn M. Marshall, Damien F. Hudson
      Pages 91-102
  4. Chromosomal Assays of SMC Activity

  5. Biochemical Assays of SMC Activity

    1. Front Matter
      Pages 167-167
    2. Rupesh Kumar, Soon Bahng, Kenneth J. Marians
      Pages 169-180
    3. Menelaos Voulgaris, Thomas G. Gligoris
      Pages 197-208
    4. Sheetal Sharma, Fabrizio d’Adda di Fagagna
      Pages 209-219
  6. Microscopy-Based Assays of SMC Activity

    1. Front Matter
      Pages 221-221
    2. Suchitha Raghunathan, Anjana Badrinarayanan
      Pages 223-238
    3. Afroze Chimthanawala, Anjana Badrinarayanan
      Pages 239-250
    4. Catarina Carmo, Margarida Araújo, Raquel A. Oliveira
      Pages 251-268 Open Access
    5. Logan R. Myler, Michael M. Soniat, Xiaoming Zhang, Rajashree A. Deshpande, Tanya T. Paull, Ilya J. Finkelstein
      Pages 269-287
  7. Theoretical Modeling and Simulation of SMC Activity

    1. Front Matter
      Pages 289-289
    2. Josh Lawrimore, Yunyan He, Gregory M. Forest, Kerry Bloom
      Pages 291-318
    3. Yuji Sakai, Tatsuya Hirano, Masashi Tachikawa
      Pages 319-334
  8. Back Matter
    Pages 335-336

About this book


This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA.

Chapters 3, 10 and 19 are available Open Access under a CC BY 4.0 license via


Chromosome organization Structural Maintenance of Chromosomes SMC proteins Bacterial models Eukaryotic models Genetic manipulation

Editors and affiliations

  • Anjana Badrinarayanan
    • 1
  1. 1.National Centre for Biological SciencesTata Institute of Fundamental Research (TIFR)BangaloreIndia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9519-6
  • Online ISBN 978-1-4939-9520-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site