Cell-Based Assays Using iPSCs for Drug Development and Testing

  • Carl-Fredrik Mandenius
  • James A. Ross

Part of the Methods in Molecular Biology book series (MIMB, volume 1994)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Zameel Cader, Martin Graf, Mark Burcin, Carl-Fredrik Mandenius, James A. Ross
    Pages 1-14
  3. Differentiation of iPC to Target Cells for Assays

    1. Front Matter
      Pages 15-15
    2. Klaus Christensen, Filip Roudnicky, Mark Burcin, Christoph Patsch
      Pages 17-29
    3. Katharina Janssen, Lamiaa Bahnassawy, Claudia Kiefer, Jürgen Korffmann, Georg C. Terstappen, Viktor Lakics et al.
      Pages 31-39
    4. Caroline Halloin, Michelle Coffee, Felix Manstein, Robert Zweigerdt
      Pages 55-70
    5. Imen Jebeniani, Shunli Ding, Michel Pucéat
      Pages 71-77
    6. Felix Manstein, Caroline Halloin, Robert Zweigerdt
      Pages 79-91
    7. Cormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, Anja Wilmes
      Pages 93-99
    8. Teresa Bluhmki, Stefanie Traub, Eva Schruf, James Garnett, Florian Gantner, Daniel Bischoff et al.
      Pages 101-115
  4. High Throughput Metabolomics and Proteomic Cell-Based Assays

    1. Front Matter
      Pages 117-117
    2. Anna Artati, Cornelia Prehn, Jerzy Adamski
      Pages 119-130
    3. Anna Baud, Wendy E. Heywood, Daniel Little, Paul Gissen, Kevin Mills
      Pages 131-139
    4. Francesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Peter Clayton, Paul Gissen
      Pages 141-147
    5. Francesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Wendy E. Heywood, Peter Clayton et al.
      Pages 149-156
  5. High Content Imaging

    1. Front Matter
      Pages 157-157
    2. Daniel Little, Christin Luft, Oliver Pezzini-Picart, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine et al.
      Pages 159-164
    3. Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, Paul Gissen
      Pages 165-174
    4. Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, Paul Gissen
      Pages 175-184
    5. Jonas Christoffersson, Florian Meier, Henning Kempf, Kristin Schwanke, Michelle Coffee, Mario Beilmann et al.
      Pages 185-193
  6. Electrophysiology Assays

  7. Miscellaneous Cell-Based Assays

    1. Front Matter
      Pages 217-217
    2. Valeria Kizner, Sandra Fischer, Benjamin Jähnke, Maximilian Naujock
      Pages 219-226
    3. Jonas Christoffersson, Carl-Fredrik Mandenius
      Pages 235-241
    4. Stefanie Traub, Ralf Heilker
      Pages 243-263
  8. Back Matter
    Pages 265-266

About this book


The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.


Human induced pluripotent cells Pharmaceuticals Hepatocytes Neuronal cells Kidney cells Cardiac cells

Editors and affiliations

  • Carl-Fredrik Mandenius
    • 1
  • James A. Ross
    • 2
  1. 1.Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM)Linköping UniversityLinköpingSweden
  2. 2.Tissue Injury and Repair GroupUniversity of EdinburghEdinburghUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9476-2
  • Online ISBN 978-1-4939-9477-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site