Antigen Processing

Methods and Protocols

  • Peter van Endert

Part of the Methods in Molecular Biology book series (MIMB, volume 1988)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Kathrin Textoris-Taube, Ulrike Kuckelkorn, Christin Beier, Peter M. Kloetzel
    Pages 15-29
  3. Mirjana Weimershaus, Irini Evnouchidou, Lenong Li, Peter van Endert, Marlene Bouvier
    Pages 31-43
  4. Simon J. Powis, Antony N. Antoniou
    Pages 83-100
  5. Devin Dersh, Jonathan W. Yewdell, Jiajie Wei
    Pages 109-122
  6. Annika Nelde, Daniel J. Kowalewski, Stefan Stevanović
    Pages 123-136
  7. Michael Ghosh, Moreno Di Marco, Stefan Stevanović
    Pages 137-147
  8. Nathalie Vigneron, Vincent Stroobant, Wenbin Ma, Alexandre Michaux, Benoît J. Van den Eynde
    Pages 159-186
  9. François A. Lemonnier
    Pages 199-215
  10. Katherine S. Forsyth, Mary M. Addison, Laurence C. Eisenlohr
    Pages 217-248
  11. Maria Embgenbroich, Jasmin Mertins, Thorsten Lang, Sven Burgdorf
    Pages 249-257
  12. Mira Tohmé, Sophia Maschalidi, Bénédicte Manoury
    Pages 289-296
  13. Sebastian Temme, Nadine Temme, Norbert Koch
    Pages 297-314
  14. Tieying Hou, Cornelia Rinderknecht, Debopam Ghosh, Andreas V. Hadjinicolaou, Robert Busch, Elizabeth D. Mellins
    Pages 315-341
  15. AeRyon Kim, Isabel Emiko Ishizuka, Isamu Z. Hartman, Yuri Poluektov, Kedar Narayan, Scheherazade Sadegh-Nasseri
    Pages 343-355
  16. Monique Gannage, Rosa Barreira da Silva, Christian Münz
    Pages 357-373
  17. Jessica Matthis, Victoria King, Helena Reijonen
    Pages 387-402
  18. Christophe Macri, Ben Fancke, Kristen J. Radford, Meredith O’Keeffe
    Pages 403-418
  19. Juan José Sáez, Ana-María Lennon-Duménil, María-Isabel Yuseff
    Pages 419-437
  20. Sebastian Zeissig, Richard S. Blumberg
    Pages 439-453
  21. Back Matter
    Pages 455-458

About this book


The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.


protease inhibitors ligand production antibodies peptide epitopes Chromatofocusing

Editors and affiliations

  • Peter van Endert
    • 1
  1. 1.INSERM U1151, Université Paris DescartesHopital Necker-Enfants MaladesParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9449-6
  • Online ISBN 978-1-4939-9450-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site