Table of contents

  1. Front Matter
    Pages i-xiii
  2. Detection, Quantification, and Isolation of Acetylated Protein Targets

    1. Front Matter
      Pages 1-1
    2. Birgit Schilling, Jesse G. Meyer, Lei Wei, Melanie Ott, Eric Verdin
      Pages 3-16
    3. Paula A. Agudelo Garcia, Miranda Gardner, Michael A. Freitas, Mark R. Parthun
      Pages 17-27
  3. Lysine Acetylation

    1. Front Matter
      Pages 47-47
    2. Catherine W. Njeri, Onyekachi E. Ononye, Lata Balakrishnan
      Pages 49-56
    3. Anahita Lashgari, Jean-Philippe Lambert, Jacques Côté
      Pages 57-77
    4. Anastasia J. Lindahl, Alexis J. Lawton, Josue Baeza, James A. Dowell, John M. Denu
      Pages 79-106
  4. Sirtuin Targets of Acetylation

    1. Front Matter
      Pages 131-131
    2. Michael B. Schultz, Conrad Rinaldi, Yuancheng Lu, João A. Amorim, David A. Sinclair
      Pages 133-149
    3. Yashira L. Negrón Abril, Irma Fernández, Robert S. Weiss
      Pages 151-171
  5. Modulation of DNA Repair by Acetylation

    1. Front Matter
      Pages 173-173
    2. Shrabasti Roychoudhury, Suravi Pramanik, Hannah L. Harris, Kishor K. Bhakat
      Pages 191-206
    3. Onyekachi E. Ononye, Catherine W. Njeri, Lata Balakrishnan
      Pages 207-224
    4. Ping-Chieh Pao, Jay Penney, Li-Huei Tsai
      Pages 225-234
  6. DNA Interactive Proteins and Processing Enzymes Affected by Acetylation

  7. Influence of Acetylation Processes on Neuroepigenetics and Tumorigenesis

    1. Front Matter
      Pages 263-263
    2. Melissa Hadley, Satish Noonepalle, Debarati Banik, Alejandro Villagra
      Pages 279-307
  8. Back Matter
    Pages 309-310

About this book


The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.


DNA2 Histone deacetylation neuroepigenetics autoimmunity tumorigenesis

Editors and affiliations

  • Robert M. Brosh, Jr.
    • 1
  1. 1.NIH Biomedical Research CenterNational Institute on Aging, National Institutes of HealthBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9433-5
  • Online ISBN 978-1-4939-9434-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site