Single Cell Methods

Sequencing and Proteomics

  • Valentina Proserpio

Part of the Methods in Molecular Biology book series (MIMB, volume 1979)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Lab Setup and Tissue Preparation

    1. Front Matter
      Pages 1-1
    2. Felipe A. Vieira Braga, Ricardo J. Miragaia
      Pages 9-21
  3. Single Cell Trancriptomic Analysis

    1. Front Matter
      Pages 23-23
    2. Josephine Bageritz, Gianmarco Raddi
      Pages 73-85
    3. Marco De Simone, Grazisa Rossetti, Massimiliano Pagani
      Pages 87-110
    4. Toby P. Aicher, Shaina Carroll, Gianmarco Raddi, Todd Gierahn, Marc H. Wadsworth II, Travis K. Hughes et al.
      Pages 111-132
    5. Kedar Nath Natarajan
      Pages 133-153
    6. Shuqiang Li, Kenneth J. Livak
      Pages 197-224
  4. Single Cell Genomic and Epigenomic Analysis

    1. Front Matter
      Pages 225-225
    2. Veronica Gonzalez-Pena, Charles Gawad
      Pages 227-234
    3. Chenxu Zhu, Yun Gao, Jinying Peng, Fuchou Tang, Chengqi Yi
      Pages 251-267
  5. Single Cell Proteomic Analysis

    1. Front Matter
      Pages 283-283
    2. Susanne Heck, Cynthia Jane Bishop, Richard Jonathan Ellis
      Pages 285-303
    3. Davide Brusa, Jean-Luc Balligand
      Pages 305-315
  6. Single Cell Multi Omic Analysis

    1. Front Matter
      Pages 317-317
    2. Iraad F. Bronner, Stephan Lorenz
      Pages 319-362
    3. Youjin Hu, Qin An, Ying Guo, Jiawei Zhong, Shuxin Fan, Pinhong Rao et al.
      Pages 363-377
  7. Single Cell Screening

    1. Front Matter
      Pages 393-393
    2. Johan Henriksson
      Pages 395-406
  8. Single Cell Live Imaging

    1. Front Matter
      Pages 407-407
    2. Toru Hiratsuka, Naoki Komatsu
      Pages 409-421
  9. Single Cell Data Analysis

    1. Front Matter
      Pages 423-423
    2. Luca Alessandrì, Maddalena Arigoni, Raffaele Calogero
      Pages 425-432
    3. Kevin Baßler, Patrick Günther, Jonas Schulte-Schrepping, Matthias Becker, Paweł Biernat
      Pages 433-455
  10. Back Matter
    Pages 457-459

About this book


This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.


PCR Drop-seq sc-ChIP-seq Crispr-Screening Fisseq

Editors and affiliations

  • Valentina Proserpio
    • 1
  1. 1.Department of Life Sciences and System BiologyUniversity of Turin, Italian Institute for Genomic Medicine, IIGM TurinTorinoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9239-3
  • Online ISBN 978-1-4939-9240-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site