Functional Disulphide Bonds

Methods and Protocols

  • Philip Hogg

Part of the Methods in Molecular Biology book series (MIMB, volume 1967)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Aster E. Pijning, Philip Hogg
    Pages 1-8
  3. Frauke Gräter, Wenjin Li
    Pages 87-113
  4. Roelof H. Bekendam, Robert Flaumenhaft
    Pages 133-148
  5. Alexander Dupuy, Freda Passam
    Pages 149-163
  6. Oskar Eriksson, Jack Stopa, Bruce Furie
    Pages 165-182
  7. Stefan G. Kathman, Alexander V. Statsyuk
    Pages 245-262
  8. Arek V. Melkonian, Nielson Weng, Brad A. Palanski, Chaitan Khosla
    Pages 263-274
  9. M. Qi, James C. Weaver, S. Rahgozar, B. Giannakopoulos, S. A. Krilis
    Pages 285-293
  10. Ivan Ho Shon, Michael P. Gotsbacher, Jennifer Guille, Divesh Kumar, Rachel Codd, Philip Hogg
    Pages 295-304
  11. Chuen Wen Tan, Morgane Bourcy, Leonardo Pasalic, Vivien M. Chen
    Pages 305-321
  12. Back Matter
    Pages 323-327

About this book


This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Functional Disulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study.


Polypeptide chains Post-translational modification Protein function Cysteine thiols Covalent drugs

Editors and affiliations

  • Philip Hogg
    • 1
  1. 1.The Centenary Institute, NHMRC Clinical Trials Centre, Sydney Medical SchoolUniversity of SydneySydneyAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9186-0
  • Online ISBN 978-1-4939-9187-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site