Mouse Models of Innate Immunity

Methods and Protocols

  • Irving C. Allen

Part of the Methods in Molecular Biology book series (MIMB, volume 1960)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Artiom Gruzdev, Greg J. Scott, Thomas B. Hagler, Manas K. Ray
    Pages 1-21
  3. Greg J. Scott, Artiom Gruzdev
    Pages 23-40
  4. Kelly Roney
    Pages 57-62
  5. Kaitlin A. Read, Michael D. Powell, Bharath K. Sreekumar, Kenneth J. Oestreich
    Pages 75-84
  6. Yee Sun Tan, Yu L. Lei
    Pages 85-91
  7. Lisa K. Kozicky, Laura M. Sly
    Pages 101-112
  8. Brittany P. Boribong, Amina Rahimi, Caroline N. Jones
    Pages 113-122
  9. Natalia Malachowa, Scott D. Kobayashi, Jamie Lovaglio, Frank R. DeLeo
    Pages 139-147
  10. Veronica M. Ringel-Scaia, Michael D. Powell, Kaitlin A. Read, Irving C. Allen, Kenneth J. Oestreich
    Pages 149-160
  11. Umesh C. S. Yadav, Kota V. Ramana
    Pages 161-168
  12. Dylan K. McDaniel, Irving C. Allen
    Pages 169-180
  13. Charles E. McGee, Christopher J. Sample, Brita Kilburg-Basnyat, Kristin A. Gabor, Michael B. Fessler, Kymberly M. Gowdy
    Pages 191-205
  14. Kristin Eden
    Pages 207-214
  15. Aaron Rothemich, Janelle C. Arthur
    Pages 215-225
  16. Catherine R. Lammert, John R. Lukens
    Pages 227-236
  17. Wei Gong, Haitao Wen
    Pages 249-255
  18. Back Matter
    Pages 257-258

About this book


This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system.


Invasive Fungal Infection CRISPR/Cas9 Immune Cell Migration Neuroinflammation Toxoplasma gondii Imaging Flow Cytometry

Editors and affiliations

  • Irving C. Allen
    • 1
  1. 1.Department of Biomedical SciencesVirginia-Maryland Regional CollegeBlacksburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9166-2
  • Online ISBN 978-1-4939-9167-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site