Microbial Metabolic Engineering

Methods and Protocols

  • Christine Nicole S. Santos
  • Parayil Kumaran Ajikumar

Part of the Methods in Molecular Biology book series (MIMB, volume 1927)

Table of contents

  1. Front Matter
    Pages i-x
  2. James Jeffryes, Jonathan Strutz, Chris Henry, Keith E. J. Tyo
    Pages 11-21
  3. Yaping Yang, Jian Wang, Ruihua Zhang, Yajun Yan
    Pages 23-35
  4. Aaron M. Love, Bradley W. Biggs, Keith E. J. Tyo, Parayil Kumaran Ajikumar
    Pages 37-45
  5. Shuobo Shi, Youyun Liang, Ee Lui Ang, Huimin Zhao
    Pages 73-91
  6. Sylvestre Marillonnet, Stefan Werner
    Pages 93-109
  7. Sandra J. Aedo, Helena R. Ma, Mark P. Brynildsen
    Pages 125-138
  8. Lynn Wong, Benjamin Holdridge, Jake Engel, Peng Xu
    Pages 155-177
  9. McKenzie L. Smith, April M. Miguez, Mark P. Styczynski
    Pages 179-189
  10. Hsien-Chung Tseng, Christine Nicole S. Santos
    Pages 191-201
  11. Whitney Hollinshead, Lian He, Yinjie J. Tang
    Pages 215-230
  12. Back Matter
    Pages 247-252

About this book


This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications.


biochemical pathways promoter libraries ribosome binding site design CLIVA genome editing

Editors and affiliations

  • Christine Nicole S. Santos
    • 1
  • Parayil Kumaran Ajikumar
    • 2
  1. 1.Manus BioCambridgeUSA
  2. 2.Manus BioCambridgeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9141-9
  • Online ISBN 978-1-4939-9142-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site