Methods and Protocols

  • David A. Lyons
  • Linde Kegel

Part of the Methods in Molecular Biology book series (MIMB, volume 1936)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Sueli Marques, David van Bruggen, Gonçalo Castelo-Branco
    Pages 1-21
  3. Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, Veronique E. Miron
    Pages 23-36
  4. Michelle S. Erwig, Dörte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer et al.
    Pages 37-63
  5. Dario Magnani, Siddharthan Chandran, David J. A. Wyllie, Matthew R. Livesey
    Pages 65-77
  6. Marie-Theres Weil, Giselheid Schulz-Ëberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schäfer, Christina Müller et al.
    Pages 79-95
  7. Matthew Swire, Charles ffrench-Constant
    Pages 111-128
  8. Sylvia Agathou, Ragnhildur Thóra Káradóttir
    Pages 141-168
  9. Linde Kegel, Maria Rubio, Rafael G. Almeida, Silvia Benito, Anna Klingseisen, David A. Lyons
    Pages 185-209
  10. Sandra Goebbels, Klaus-Armin Nave
    Pages 249-274
  11. Jaime Eugenin von Bernhardi, Leda Dimou
    Pages 275-294
  12. John N. Mariani, Lisa Zou, Steven A. Goldman
    Pages 311-331
  13. Marie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Boguslawa Sadowski, Wiebke Möbius
    Pages 343-375
  14. Christopher E. McMurran, Chao Zhao, Robin J. M. Franklin
    Pages 377-396
  15. Cassandra Sampaio-Baptista, Kata Diosi, Heidi Johansen-Berg
    Pages 397-407
  16. Back Matter
    Pages 409-411

About this book


This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.


Transcriptomic profiling axons behavioral assays myelination nervous system

Editors and affiliations

  • David A. Lyons
    • 1
  • Linde Kegel
    • 2
  1. 1.Centre for Discovery Brain SciencesUniversity of EdinburghEdinburghUK
  2. 2.Centre for Discovery Brain SciencesUniversity of EdinburghEdinburghUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9070-2
  • Online ISBN 978-1-4939-9072-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site