Viral Vectors for Gene Therapy

Methods and Protocols

  • Fredric P. Manfredsson
  • Matthew J. Benskey

Part of the Methods in Molecular Biology book series (MIMB, volume 1937)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Matthew J. Benskey, Ivette M. Sandoval, Kathryn Miller, Rhyomi L. Sellnow, Aysegul Gezer, Nathan C. Kuhn et al.
      Pages 3-26
  3. Novel Modes of Gene Therapy (Going Beyond Overexpression and Knockdown)

  4. Viral Vectors

    1. Front Matter
      Pages 89-89
    2. Quentin Sandro, Karima Relizani, Rachid Benchaouir
      Pages 91-99
    3. Ivette M. Sandoval, Nathan M. Kuhn, Fredric P. Manfredsson
      Pages 101-124
    4. Shuang Hu, Mingjie Li, Ramesh Akkina
      Pages 125-134
    5. Stuart Tinch, Kathy Szczur, William Swaney, Lilith Reeves, Scott R. Witting
      Pages 135-153
    6. Ekramy E. Sayedahmed, Rashmi Kumari, Suresh K. Mittal
      Pages 155-175
    7. Andranik Kahramanian, Toshihiko Kuroda, Hiroaki Wakimoto
      Pages 177-188
  5. Viral Vector Delivery

    1. Front Matter
      Pages 211-211
    2. Sharon C. Cunningham, Ian E. Alexander
      Pages 213-219
    3. Kevin Isgrig, Wade W. Chien
      Pages 221-226
    4. C. J. Rocca, S. Cherqui
      Pages 227-234
    5. Koji Hosaka, Fredric P. Manfredsson, Brian L. Hoh
      Pages 259-265
    6. Francisco Martín, María Tristán-Manzano, Noelia Maldonado-Pérez, Sabina Sánchez-Hernández, Karim Benabdellah, Marién Cobo
      Pages 267-280
    7. Lakmini P. Wasala, Chady H. Hakim, Yongping Yue, N. Nora Yang, Dongsheng Duan
      Pages 281-294
    8. Kelsey R. Pflepsen, Cristina D. Peterson, Kelley F. Kitto, Lucy Vulchanova, George L. Wilcox, Carolyn A. Fairbanks
      Pages 305-312
    9. Lluis Samaranch, Kousaku Ohno, Waldy San Sebastian, Krystof Bankiewicz
      Pages 313-324
  6. Back Matter
    Pages 325-328

About this book


This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments.


Recombinant viral vectors Gene expression manipulation Genetic modification Vector delivery Pharmacology

Editors and affiliations

  • Fredric P. Manfredsson
    • 1
  • Matthew J. Benskey
    • 2
  1. 1.Translational Science and Molecular MedicineMichigan State UniversityGrand RapidsUSA
  2. 2.Translational Science and Molecular MedicineMichigan State UniversityGrand RapidsUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9064-1
  • Online ISBN 978-1-4939-9065-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site