Methods and Protocols

  • Petros Papagerakis

Part of the Methods in Molecular Biology book series (MIMB, volume 1922)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Protocols for Establishment of Dental Cells Lines and Animal Models

  3. Protocols for Dental Stem Cells and Tissue Regeneration

    1. Front Matter
      Pages 57-57
    2. Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Frédéric Mallein-Gerin et al.
      Pages 77-90
    3. Qing Dong, Yuanyuan Wang, Fatemeh Mohabatpour, Li Zheng, Silvana Papagerakis, Daniel Chen et al.
      Pages 91-101
    4. R. C. Babb, D. Chandrasekaran, L. K. Zaugg, P. T. Sharpe
      Pages 111-119
    5. Petros Kechagioglou, Eleftherios Andriotis, Petros Papagerakis, Silvana Papagerakis
      Pages 121-128
    6. Kaushik Mukherjee, Qichao Ruan, Janet Moradian-Oldak
      Pages 129-138
    7. Elizabeth E. Smith, Pamela C. Yelick
      Pages 139-150
  4. Protocols for Studying Gene and Protein Expression

    1. Front Matter
      Pages 161-161
    2. Jingyi Wu, Jian Q. Feng, Xiaofang Wang
      Pages 163-171
    3. D. Hotton, A. Berdal, A. Bolaños
      Pages 173-180
    4. Jingyi Wu, Xiaofang Wang
      Pages 211-218
    5. Jingtan Su, Rucha Arun Bapat, Janet Moradian-Oldak
      Pages 229-236
  5. Protocols for Biochemistry and Imaging

  6. Protocols to Study Dental Diseases

    1. Front Matter
      Pages 333-333
    2. Bennett T. Amaechi, Livia M. A. Tenuta, Antonio P. Ricomini Filho, Jaime A. Cury
      Pages 357-368
    3. Basma Sulaiman Ghandourah, Anna Lefkelidou, Raed Said, Xanthippi Chatzistavrou, Susan Flannagan, Carlos Gonzáles-Cabezas et al.
      Pages 369-377
    4. June Hsiao, Yuanyuan Wang, Li Zheng, Ruirui Liu, Raed Said, Lubomir Hadjiyski et al.
      Pages 393-403
  7. Protocols for Genetic, Epigenetic and Clinical Studies

    1. Front Matter
      Pages 405-405
    2. Tristan Rey, Julien Tarabeux, Bénédicte Gerard, Marion Delbarre, Antony Le Béchec, Corinne Stoetzel et al.
      Pages 407-452
    3. Bruna Rabelo Amorim, Pollyanna Almeida Costa dos Santos, Caroline Lourenço de Lima, Denise Carleto Andia, Juliana Forte Mazzeu, Ana Carolina Acevedo
      Pages 453-492
    4. Cary S. Agler, Dmitry Shungin, Andrea G. Ferreira Zandoná, Paige Schmadeke, Patricia V. Basta, Jason Luo et al.
      Pages 493-509
    5. Jeannie Ginnis, Andrea G. Ferreira Zandoná, Gary D. Slade, John Cantrell, Mikafui E. Antonio, Bhavna T. Pahel et al.
      Pages 511-523
    6. Petros Papagerakis, Li Zheng, Doohak Kim, Raed Said, Amber A. Ehlert, Kevin K. M. Chung et al.
      Pages 549-562
  8. Back Matter
    Pages 563-565

About this book


This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva,  and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth.


epithelial cells mesenchymal cells dentin knock-out mouse lines Dentistry

Editors and affiliations

  • Petros Papagerakis
    • 1
  1. 1.School of DentistryUniversity of MichiganAnn ArborUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9011-5
  • Online ISBN 978-1-4939-9012-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site