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Hepatic Stem Cells

Methods and Protocols

  • Naoki Tanimizu

Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Isolation of Progenitor Cells

    1. Front Matter
      Pages 1-1
    2. Atsunori Tsuchiya, Shuji Terai
      Pages 3-8
    3. Bin Li, Craig Dorrell, Pamela S. Canady, Leslie Wakefield
      Pages 19-27
    4. Junichi Kino, Norihisa Ichinohe, Masayuki Ishii, Toshihiro Mitaka
      Pages 29-41
  3. Characterization of Liver Progenitors In Vivo

    1. Front Matter
      Pages 43-43
    2. Teresa Rubio-Tomás, Beatriz Aguilar-Bravo, Pau Sancho-Bru
      Pages 45-57
  4. Generation of Hepatocytes, Cholangiocytes, and Their Progenitors

    1. Front Matter
      Pages 91-91
    2. Pengyu Huang, Lulu Sun, Ludi Zhang, Lijian Hui
      Pages 93-101
    3. Guangqi Song, Qinggong Yuan, Zhen Dai, Hsin-Chieh Tsay, Xizhong Shen, Michael Ott et al.
      Pages 103-115
    4. Taketomo Kido, Yuta Koui
      Pages 131-142
    5. Akihide Kamiya, Kazuya Anzai, Kota Tsuruya, Hiromi Chikada
      Pages 143-153
  5. Reconstitution of Liver Tissue Structures

  6. Liver Injury Models

    1. Front Matter
      Pages 199-199
    2. Taro Takami, Kenji Tani, Yasuho Taura, Isao Sakaida
      Pages 201-209
    3. Sei Kakinuma, Akihide Kamiya
      Pages 211-219
    4. Hayato Nakagawa, Nobumi Suzuki, Kazuhiko Koike
      Pages 237-245
  7. Back Matter
    Pages 247-248

About this book

Introduction

This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology.

Keywords

biliary stem cells hepatocyte progenitors liver epithelial cells liver regeneration fibroblasts

Editors and affiliations

  • Naoki Tanimizu
    • 1
  1. 1.Sapporo Medical UniversitySapporoJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8961-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8960-7
  • Online ISBN 978-1-4939-8961-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site